Báo cáo khoa học: Reaction mechanisms of thiamin diphosphate enzymes: defining states of ionization and tautomerization of the cofactor at individual steps
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We summarize the currently available information regarding the state of ionization and tautomerization of the 4¢-aminopyrimidine ring of the thia-mine diphosphate on enzymes requiring this coenzyme. This coenzyme forms a series of covalent intermediates with its substrates as an electro-philic catalyst, and the coenzyme itself also carries out intramolecular pro-ton transfers, which is virtually unprecedented in coenzyme chemistry.
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