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INTRODUCTION
1. The urgency of the thesis
Today, the economic development, the need for care and protection of the
health of the community becomes more urgent. Thus, the demand for medicine
in preventing and treatment of incurable diseases especially cancer is
increasing.
The plant resources in Viet Nam are rich and multiple with over 12000
species of 309 plant families. Among them, 3900 species have been used for
medicine. However, the relationship between the chemical structure and
pharmacological activity in many cases has been not clarified.
Allophylus livescens and Chirita halongesis, two endemic species of Ha
Long Bay, have not yet studied on chemical compositions and biological
activities. Oldenlandia pinifolia is used in traditional medicine for treatment of
inflammation, pain and other diseases and there has
been only one report on the phytochemistry of this species.
To contribute to understanding, research and systematic evaluation of
biologically active compounds from coastal plants and mangroves, and to
propose directions for the exploitation and conservation of genetic resources of
the rare plant species in Vietnam, we conducted research on the chemical
constituents and cytotoxic activity of 3 species: Allophylus livescens Gagnep.,
Chirita halongesis Kiew & T. H. Nguyen and Oldenlandia pinifolia (Wall. ex
G. Don) Kuntze growing in Viet Nam.
2. The objectives of the thesis
Study on the chemical compositions of three species: Allophylus
livescens, Chirita halongesis and Oldenlandia pinifolia growing in Viet Nam to
find bioactive compounds as the basis for further research.
3. The main contents of the thesis
- Isolation of compounds from three species Allophylus livescens, Chirita
halongesis and Oldenlandia pinifolia collected in Viet Nam by column
chromatography.
- Determination of chemical structure of isolated compounds based on
IR, MS, 1D-NMR, 2D-NMR spectroscopic analysis.
- Evaluation of cytotoxic activity of extracts and some isolated
compounds.
4. The contribution of the thesis
This is the first time the chemical constituents and cytotoxic activities of
Allophylus livescens and Chirita halongenis species have been studied in
Vietnam as well as in the world. Two compounds were first isolated from
Allophylus genus: 1,6,10,14-phytatetraen-3-ol and catechin. Oleanolic acid,
decaffeoylacetoside and β-hydroxy acteoside were found for the first time in
Chirita genus.
This is the first study on the cytotoxic activity of Oldenlandia pinofolia
