Ứng dụng máy đo phản xạ nhiệt Tail-Flick Unit 37360 trong thử tác dụng giảm đau của Efferalgan Codein trên động vật thực nghiệm

Pain management remains a critical area in pharmacology, necessitating robust and standardized methods for assessing analgesic efficacy. The "Tail-Flick" test, utilizing "thermal reflex" stimulation, is widely recognized for its simplicity, speed, and ability to minimize individual variability in evaluating central "analgesia". Despite the availability of the Tail-Flick Unit 37360 at institutions like Hai Duong Central College of Pharmacy, its practical application in teaching and research has been limited. This study addresses this gap by developing a standardized protocol for the Tail-Flick Unit 37360, aiming to enhance its utility. Furthermore, it seeks to apply this established protocol to investigate the "analgesic effect" of "Efferalgan Codeine" in experimental animals, thereby contributing to both academic training and pharmacological research.

Pharmacology students and educators, pharmaceutical researchers, and scientists involved in preclinical drug testing and pain management.

This study aimed to establish a standardized protocol for operating the "Tail-Flick" Unit 37360 and apply it to assess the "analgesic effect" of "Efferalgan Codeine" in experimental animals. A meticulously designed experimental study was conducted on Swiss albino mice, where their "thermal reflex" nociceptive responses were measured using the Tail-Flick Unit 37360 before and after administration of the test substance. The methodological framework involved a five-step procedure, including device initialization, optimal infrared (IR) intensity calibration (found to be 30 for stable results), meticulous animal preparation, precise measurement of pain reaction latency, and post-experiment equipment maintenance. Statistical analysis using ANOVA and t-tests compared the drug's effect against a control group. The findings successfully established a robust and standardized operational procedure for the Tail-Flick Unit 37360. Crucially, "Efferalgan Codeine", administered at a dose equivalent to 7.2 mg/kg Codeine, demonstrated a significant prolongation of pain reaction latency at both 0.5 hour and 1 hour following administration. However, this pronounced "analgesia" diminished by 2.5 hours, which the discussion attributed to potential decreases in drug concentration or increased tail sensitivity due to repeated "thermal reflex" exposure. The study concludes that the Tail-Flick Unit 37360 is an effective and accurate tool for evaluating central "analgesic" activity, offering advantages in terms of speed, cost-effectiveness, and reproducibility. The developed experimental protocol serves as a valuable resource, ready for integration into pharmacology training curricula, thereby enhancing practical skills and research capabilities in pain management.