Nghiên cứu độc tính cấp và độc tính bán trường diễn của dung dịch xịt hỗ trợ điều trị tổn thương hở ở da từ nano bạc và một số dược liệu

Open skin lesions represent a significant clinical challenge, often associated with chronic conditions and impaired mobility. Traditional treatments for these wound injuries can lead to discomfort, delayed healing, and the emergence of antibiotic resistance, highlighting an urgent need for innovative and safe alternatives. This study addresses this need by evaluating the safety profile of a novel spray solution formulated from nanosilver and a blend of herbal medicines (Coptis chinensis, Fibraurea recisa, Lonicera japonica, Eclipta prostrata, and Curcuma longa). The primary objective is to assess its acute toxicity and subchronic toxicity, providing crucial data for its potential therapeutic application in promoting wound healing.

Researchers in toxicology, pharmacology, dermatology, wound care, and traditional medicine, as well as pharmaceutical developers interested in novel topical formulations for skin lesions.

This research systematically investigated the safety of a novel spray solution designed for treating wound injuries on the skin, integrating nanosilver with a combination of herbal medicines. The study employed a two-pronged approach, evaluating both acute toxicity and subchronic toxicity in animal models according to established guidelines. For acute toxicity assessment, 40 white rats were administered the solution, with results indicating no adverse effects even at the highest dose of 2000 mg/kg, suggesting a favorable immediate safety profile. The subchronic toxicity study involved 30 rabbits, treated topically with the spray solution at 500 mg/kg daily for 28 days. Comprehensive evaluations during this period, including body weight changes, hematological parameters (platelets, red blood cells, leukocyte counts), and key biochemical markers for liver and kidney function (AST, ALT, urea, creatinine, cholesterol, bilirubin, total protein), revealed no significant statistical differences compared to control groups. Furthermore, histological examination of liver and kidney tissues showed no pathological alterations. These findings robustly demonstrate that the formulation did not exhibit any signs of acute toxicity or adverse subchronic effects on vital organ functions and hematological indices. This comprehensive safety evaluation underscores the significant potential of this nanosilver and herbal medicines-based spray solution as a safe and effective adjunctive therapy for open skin lesions, paving the way for its further clinical development and application.