
Molecular Biotechnology
Tran Ngoc Duc, PhD
VIETNAM NATIONAL UNIVERSITY AT HO CHI MINH CITY
INTERNATIONAL UNIVERSITY

Basic techniques in DNA
technology

1. Polymerase chain reaction (PCR)
2. Gel electrophoresis
3. Primer Design
4. RT-PCR
5. Digestion
6. Southern blot/Northern blot
7. Detectable signal based techniques
8. Site-directed mutagenesis

Polymerase Chain Reaction is widely held as one of
the most important inventions of the 20th century in
molecular biology. Small amounts of the genetic material
can now be amplified to be able to a identify, manipulate
DNA, detect infectious organisms, including the viruses
that cause AIDS, hepatitis, tuberculosis, detect genetic
variations, including mutations, in human genes and
numerous other tasks.


