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Thuyết trình bảo vệ khóa luận

Chuyên đề: Hệ thống bảo vệ so lệch thanh cái

Chuyên đề: Hệ thống bảo vệ so lệch thanh cái

Chuyên đề: Hệ thống bảo vệ so lệch thanh cái có kết cấu nội dung gồm 4 phần. Trong đó Phần 1 giới thiệu chung, phần 2 giới thiệu về nguyên lý làm việc của bảo vệ so lệch, phần 3 giới thiệu vệ bảo vệ trở kháng cao và thấp, phần 4 giới thiệu một số bảo vệ được sử dụng phổ biến trong thực tế.
15 trang
909 lượt xem
151 lượt tải
Tóm tắt Khóa luận: Bảo đảm ATTT trong kiểm soát truy nhập

Tóm tắt Khóa luận: Bảo đảm ATTT trong kiểm soát truy nhập

Tóm tắt Khóa luận: Bảo đảm ATTT trong kiểm soát truy nhập tập trung vào một số phương pháp kiểm soát truy nhập, một số chính sách truy cập, và một số kĩ thuật kiểm soát truy nhập. Mời các bạn cùng tìm hiểu và tham khảo nội dung thông tin tài liệu.
10 trang
147 lượt xem
16 lượt tải
Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction

Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction

In this study, we isolated a 25-kDa novel snake venom protein, designated ablomin, from the venom of the Japanese Mamushi snake (Agkistrodon blomhoffi). The amino-acid sequence of this protein was determined by peptide sequencing and cDNA cloning. The deduced sequence showed high similarity to helothermine from the Mexican beaded lizard (Heloderma horridum horridum), which blocks voltage-gated calcium and potassium channels, and ryanodine receptors. Ablomin blocked contraction of rat tail arterial smooth muscle elicited by high K+-induced depolarization in the 0.1–1 lM range, but did not block caffeine-stimulated contraction....
8 trang
67 lượt xem
3 lượt tải
Báo cáo khoa học: A peptide derived from cyclin-dependent kinase activator (p35) specifically inhibits Cdk5 activity and phosphorylation of tau protein in transfected cells

Báo cáo khoa học: A peptide derived from cyclin-dependent kinase activator (p35) specifically inhibits Cdk5 activity and phosphorylation of tau protein in transfected cells

Cyclin-dependent kinase-5 (Cdk5) is a serine/threonine kinase activated by its neuron-specific activator, p35, or its truncated form, p25. It has been proposed that the deregu-lation of Cdk5 activity by association with p25 in human brain tissue disrupts the neuronal cytoskeleton and may be involved in neurodegenerative diseases such as Alzheimer’s disease. In this study, we demonstrate that a short peptide (amino acid residues 154–279; Cdk5 inhibitory peptide; CIP), derived from p35, specifically inhibits Cdk5 activity in vitro and in HEK293 cells cotransfected with the peptide and Cdk5/p25, but had no effect on endogenous cdc2 kinase activity....
8 trang
50 lượt xem
2 lượt tải
Báo cáo khoa học: Two conserved domains in regulatory B subunits mediate binding to the A subunit of protein phosphatase 2A

Báo cáo khoa học: Two conserved domains in regulatory B subunits mediate binding to the A subunit of protein phosphatase 2A

Protein phosphatase 2A (PP2A) is an abundant heterotri-meric serine/threonine phosphatase containing highly con-served structural (A) and catalytic (C) subunits. Its diverse functions in the cell are determined by its association with a highly variable regulatory and targeting B subunit. At least three distinct gene families encoding B subunits are known: B/B55/CDC55, B¢/B56/RTS1 and B¢¢/PR72/130. No homologyhasbeen identi®edamong theBfamilies, and little is knownabout howtheseBsubunits interactwith the PP2A A and C subunits....
7 trang
43 lượt xem
3 lượt tải
Báo cáo Y học: The role of the second binding loop of the cysteine protease inhibitor, cystatin A (stefin A), in stabilizing complexes with target proteases is exerted predominantly by Leu73

Báo cáo Y học: The role of the second binding loop of the cysteine protease inhibitor, cystatin A (stefin A), in stabilizing complexes with target proteases is exerted predominantly by Leu73

The aimof this workwas to elucidate the roles of individual residues within the flexible second binding loop of human cystatin A in the inhibition of cysteine proteases. Four recombinant variants of the inhibitor, each with a single mutation, L73G, P74G, Q76G or N77G, in the most exposed part of this loopwere generated by PCR-based site-directed mutagenesis. The binding of these variants to papain, cathepsin L, and cathepsin B was characterized by equilibrium and kinetic methods.
10 trang
51 lượt xem
2 lượt tải
Báo cáo khoa học: Solution structure of the matrix attachment region-binding domain of chicken MeCP2

Báo cáo khoa học: Solution structure of the matrix attachment region-binding domain of chicken MeCP2

Methyl-CpG-binding protein 2 (MeCP2) is a multifunc-tional protein involved in chromatin organization and silencing of methylated DNA. MAR-BD, a 125-amino-acid residue domain of chicken MeCP2 (cMeCP2, origin-ally named ARBP), is the minimal protein fragment required to recognize MAR elements and mouse satellite DNA. Here we report the solution structure of MAR-BD as determined by multidimensional heteronuclear NMR spectroscopy.
8 trang
46 lượt xem
2 lượt tải
Báo cáo khoa học: Role of CCP2 of the C4b-binding protein b-chain in protein S binding evaluated by mutagenesis and monoclonal antibodies

Báo cáo khoa học: Role of CCP2 of the C4b-binding protein b-chain in protein S binding evaluated by mutagenesis and monoclonal antibodies

Complement regulator C4b-binding protein (C4BP) and the anticoagulant vitamin K-dependent protein S form a high affinity complexin human plasma. C4BP is composed of sevena-chains and a uniqueb-chain, each chain comprising repeating complement control protein (CCP) modules. The binding site for protein S mainly involves the first of the threeb-chain CCPs (CCP1). However, recently it has been suggested that CCP2 of theb-chain also contributes to the binding of proteinS.
8 trang
48 lượt xem
2 lượt tải
Một số nghiên cứu về hàm băm và giao thức mật mã

Một số nghiên cứu về hàm băm và giao thức mật mã

Giao thức mật mã (hay giao thức an toàn) là các giao thức (trên lý thuyết hoặc đã thực hiện) nhằm thực hiện các chức năng liên quan tới bảo mật bằng các kỹ thuật mật mã.
152 trang
150 lượt xem
35 lượt tải
Báo cáo khoa học: AtCYS1, a cystatin from Arabidopsis thaliana, suppresses hypersensitive cell death

Báo cáo khoa học: AtCYS1, a cystatin from Arabidopsis thaliana, suppresses hypersensitive cell death

In plants, cysteine protease inhibitors are involved in the regulation of protein turnover and play an important role in resistance against insects and pathogens. AtCYS1from Arabidopsis thalianaencodes a protein of 102 amino acids that contains the conserved motif of cysteine protease inhibitors belonging to the cystatin superfamily (Gln-Val-Val-Ala-Gly). Recombinant A. thaliana cystatin-1 (AtCYS1) was expressed in Escherichia coliand purified.
12 trang
42 lượt xem
3 lượt tải
Báo cáo khoa học: Caspase-2 is resistant to inhibition by inhibitor of apoptosis proteins (IAPs) and can activate caspase-7

Báo cáo khoa học: Caspase-2 is resistant to inhibition by inhibitor of apoptosis proteins (IAPs) and can activate caspase-7

Caspases are a family of cysteine proteases with roles in cytokine matur-ation or apoptosis. Caspase-2 was the first pro-apoptotic caspase identified, but its functions in apoptotic signal transduction are still being elucidated. This study examined the regulation of the activity of caspase-2 using recombinant proteins and a yeast-based system. Our data suggest that for human caspase-2 to be active its large and small subunits must be separ-ated. For maximal activity its prodomain must also be removed....
14 trang
44 lượt xem
4 lượt tải
Báo cáo khoa học: Entamoeba histolytica TATA-box binding protein binds to different TATA variants in vitro

Báo cáo khoa học: Entamoeba histolytica TATA-box binding protein binds to different TATA variants in vitro

The ability ofEntamoeba histolyticaTATA binding protein (EhTBP) to interact with different TATA boxes in gene promoters may be one of the key factors to perform an efficient transcription in this human parasite. In this paper we used several TATA variants to study the in vitro EhTBP DNA-binding activity and to determine the TATA-EhTBP dissociation constants. The presence of EhTBP in complexes formed by nuclear extracts (NE) and the TATTTAAA oligonucleotide, which corresponds to the canonical TATA box for E. histolytica, was demonstrated by gel-shift assays. ...
13 trang
73 lượt xem
2 lượt tải
Báo cáo khoa học: CpG methylation of the CENP-B box reduces human CENP-B binding

Báo cáo khoa học: CpG methylation of the CENP-B box reduces human CENP-B binding

In eukaryotes, CpG methylation is an epigenetic DNA modification that is important for heterochromatin formation. Centromere protein B (CENP-B) specifically binds to the centromeric 17 base-pair CENP-B box DNA, which contains two CpG dinucleotides. In this study, we tested complex formation by the DNA-binding domain of CENP-B with methylated and unmethylated CENP-B box DNAs, and found that CENP-B preferentially binds to the unmethylated CENP-B box DNA.
8 trang
38 lượt xem
5 lượt tải
Báo cáo khoa học: Two splicing isoforms of the Y-box protein ctYB-1 appear on the same mRNA molecule

Báo cáo khoa học: Two splicing isoforms of the Y-box protein ctYB-1 appear on the same mRNA molecule

Y-box proteins constitute an evolutionarily conserved family of DNA- and RNA-binding proteins involved in the regulation of transcription and translation. In the dipteran Chironomus tentans, a homologue to the verte-brate Y-box protein YB-1 was recently characterized and designated ctYB-1. It is transferred from nucleus to cytoplasm bound to mRNA and is likely to affect translation.
10 trang
42 lượt xem
4 lượt tải
Báo cáo khoa học: Specific membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member

Báo cáo khoa học: Specific membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member

I C , an endogenous cytoplasmic inhibitor of vacuolar carboxypeptidase Y in the yeast Saccharomyces cerevisiae, is classified as a member of the phos-phatidylethanolamine-binding protein family. The binding of I C to phos-pholipid membranes was first analyzed using a liposome-binding assay and by surface plasmon resonance measurements, which revealed that the affin-
10 trang
56 lượt xem
4 lượt tải
Báo cáo khoa học: Activity of matrix metalloproteinase-9 against native collagen types I and III

Báo cáo khoa học: Activity of matrix metalloproteinase-9 against native collagen types I and III

Interstitial collagen types I, II and III are highly resistant to proteolytic attack, due to their triple helical structure, but can be cleaved by matrix metalloproteinase (MMP) collagenases at a specific site, approximately three-quarters of the length from the N-terminus of each chain.
10 trang
43 lượt xem
4 lượt tải
Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas

Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas

Chitinase-like proteins have been identified in insects and mammals as non-enzymatic members of the glycoside hydrolase family 18. Recently, the first molluscan chitinase-like protein, namedCrassostrea gigas(Cg)-Clp1, was shown to control the proliferation and synthesis of extracellular matrix components of mammalian chondrocytes.
9 trang
60 lượt xem
4 lượt tải
Báo cáo khoa học: The Janus-faced atracotoxins are specific blockers of invertebrate KCa channels

Báo cáo khoa học: The Janus-faced atracotoxins are specific blockers of invertebrate KCa channels

The Janus-faced atracotoxins are a unique family of excitatory peptide toxins that contain a rare vicinal disulfide bridge. Although lethal to a wide range of invertebrates, their molecular target has remained enigmatic for almost a decade.
15 trang
64 lượt xem
4 lượt tải

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