Fungus

Red yeast rice is produced from rice using Monascus purpureus fungus, which results in the red colour of the fermented rice. Red yeast rice has been used for a thousand years as a food preservative and in traditional medicine to support vascular and digestive health. This study used Liquid Chromatography-Mass Spectrometry (LC-MS/MS) to concurrently analyse citrinin and monacolin K produced by M. purpureus strain.

11p viaburame 14-03-2025 1 1   Download

Viêm phổi có thể do biến chứng từ bệnh sởi, ho gà, cúm, viêm phế quản, hen hoặc bất kì bệnh nặng nào khác [1]. Trong các bệnh sưng phổi, nhiều nhất là do vi trùng Pneumococcus. Ngoài ra còn có thể do vi trùng (bacteria) khác, siêu vi trùng (virus), có khi do ký sinh trùng (parasites), hoặc loài nấm (fungus).

55p buddy7 29-06-2011 234 42   Download

The research herein builds on the work of Rojek (2003) and further investigates the bioremediation of NOM wastes. The aim of this research was to evaluate the effectiveness of selected white-rot fungi and yeast for the biodegradative removal of concentrated NOM from solution. The effectiveness of the white-rot fungus P. chrysosporium ATCC 34541 for the biological degradation of different batches of MIEXTM NOM concentrate and the effects of different characteristics of the NOM on the process and thus the resistance of the NOM to microbial removal were investigated.

131p runthenight05 01-03-2023 13 3   Download

This history of antimicrobials is marked with impressive discoveries, the majority of which have their origin in natural products. However in this work, the antimicrobial activity of β- nitrostyrenes and related compounds of synthetic origin is reviewed and investigated with a particular focus on the influence of fluorine functionality. Various fluorinated β-methyl-β-nitrostyrene compounds were prepared, their minimum inhibition concentration in cultures of Gram positive, Gram negative and a fungus and their lipophilicity was determined.

133p runthenight05 01-03-2023 10 3   Download

Morphological and cultural variability of S. rolfsii infecting tomato were studied on different solid and liquid media based on radial growth, topography, sclerotial number, diameter of sclerotia, test weight of sclerotial bodies and dry mycelial weight. Significant variability with reference to mycelial and sclerotial characters was observed on different media. This investigation revealed that the maximum mycelial growth was observed in Potato Dextrose Agar (9 cm) and more test weight (262 mg) of sclerotial bodies was recorded in Sabouraud’s dextrose agar.

8p chauchaungayxua6 26-06-2020 10 3   Download

The expression of mitochondrial and hydrogenosomal ADP/ATP carriers (AACs) from plants, rat and the anaerobic chytridiomycete fungus Neocallimastix spec. L2 in Escherichia coli allows a functional integration of the recombinant proteins into the bacterial cytoplasmic membrane. For AAC1 and AAC2 from rat, apparent Km values of about 40 lM for ADP, and 105 lM or 140 lM, respectively, for ATP have been determined, similar to the data reported for isolated rat mitochondria.

10p system191 01-06-2013 39 4   Download

Quercetin 2,3-dioxygenase (2,3QD) is a copper-containing dioxygenase that catalyses the oxidation of the flavonol quercetin to 2-protocatechuoylphloroglucinol carboxylic acid with concomitant production of carbon monoxide. In contrast to iron dioxygenases, very little is known about copper dioxygenases. We have characterized 2,3QD from the fungus Aspergillus japonicus by electron paramagnetic resonance spectroscopy (EPR). At pH 6.0, 2,3QD shows a mixture of two EPR species. The major form has parameters typical of type 2 Cu sites (g// ¼ 2.330, A// ¼ 13.

9p system191 01-06-2013 33 7   Download

Manganese lipoxygenase was isolated to homogeneity from the take-all fungus, Gaeumannomyces graminis. The C-terminal amino acids and several internal peptides were sequenced, and the information was used to obtain a cDNA probe by RT/PCR. Screening of a genomic library of G. graminis yielded a full-length clone of the Mn-Lipoxygenase gene. cDNA analysis showed that the gene spanned 2.6 kb and contained one intron (133 bp). Northern blot analyses indicated two transcripts (2.7 and 3.1 kb). The deduced amino-acid sequence of the Mn-Lipoxygenase precursor (618 amino acids, 67.

8p system191 01-06-2013 40 4   Download

Intracellular pH homeostasis in the filamentous fungus Aspergillus niger was measured in real time by 31P NMR during perfusion in the NMR tube of fungal biomass immobilized in Ca2+-alginate beads. The fungus maintained constant cytoplasmic pH (pHcyt) and vacuolar pH (pHvac) values of 7.6 and 6.2, respectively, when the extracellular pH (pHex) was varied between 1.5 and 7.0 in the presence of citrate. Intracellular metabolism did not collapse until a DpH over the cytoplasmic membrane of 6.6–6.7 was reached (pHex 0.7–0.8).

10p research12 01-06-2013 42 4   Download

Cytochrome P450foxy (P450foxy, CYP505) is a fused protein of cytochrome P450 (P450) and its reductase isolated from the fungus Fusarium oxysporum, which catalyzes the subterminal (x-1x-3) hydroxylation of fatty acids. Here, we produced, purified and characterized a fused recombinant protein (rP450foxy) using the Escherichia coli expression system. Purified rP450foxy was catalytically and spectrally indistinguishable from the native protein, but most of the rP450foxy was recovered in the soluble fraction of E. coli cells unlike the membrane-bound native protein....

8p research12 01-06-2013 62 4   Download

The Phycomyces blakesleeanus wild-type is yellow, because it accumulates b-carotene as the main carotenoid. A new carotenoid mutant of this fungus (A486) was isolated, after treatment with ethyl methane sulfonate (EMS), showing a whitish coloration. It accumulates large amounts of phytoene, small quantities of phyto¯uene, f-carotene and neurosporene, in decreasing amounts, and traces of b-carotene. This phenotype indicates that it carries a leaky mutation a€ecting the enzyme phytoene dehydrogenase (EC 1.3.-.-), which is speci®ed by the gene carB....

7p research12 01-06-2013 48 5   Download

Diagnostic tests by molecular biology is made for studying the relations among Fusarium species for linking production of proteins, degree of relationship and occurrence of malformation. Determination of proteins for isolates causing-disease by SDS-PAGE explained there’s specific band for each fungus and there are common bands among some isolates of fungi. Since, band with MW 30 KDa represented only in F. proliferatum and F. oxysporum and F. subgluti-nans respectively. This band considered as specific band for these isolates, which released high patho-genisity effect.

7p zingzing09 24-04-2013 59 3   Download

Plant cell wall proteins called expansins are thought to disrupt hydrogen bonding between cell wall polysaccha-rides without hydrolyzing them. We describe here a novel gene with sequence similarity to plant expansins, isolated from the cellulolytic fungus Trichoderma reesei. The pro-tein named swollenin has an N-terminal fungal type cel-lulose binding domain connected by a linker region to the expansin-like domain.

10p research12 23-04-2013 36 4   Download

The major cellulase secreted by the filamentous fungus Trichoderma reeseiis cellobiohydrolase Cel7A. Its three-dimensional structure has been solved and various mutant enzymes produced. In order to study the potential use of T. reeseiCel7A in the alkaline pHrange, the thermal sta-bilityofCel7Awas studiedas a functionof pHwith thewild-type and two mutant enzymes using different spectroscopic methods. Tryptophan fluorescence and CD measurements of the wild-type enzyme show an optimal thermostability between pH3.5–5.

8p tumor12 20-04-2013 40 4   Download

A new component of theb-1,6-glucanase (EC 3.2.1.75) multienzymatic complex secreted by Trichoderma harzianumhas been identified and fully characterized. The protein, namely BGN16.3, is the third isozyme display-ing endo-b-1,6-glucanase activity described up to now in T. harzianum CECT 2413. BGN16.3 is an acidicb-1,6-glucanase that is specifically induced by the presence of fungal cell walls in T. harzianumgrowth media.

8p fptmusic 11-04-2013 33 2   Download

Aspergillus fumigatusis responsible for many allergic respiratory diseases, the most notable of which )due to its severity)is allergic bronchopulmo-nary aspergillosis. Aspf1 is a major allergen of this fungus: this 149-amino acid protein belongs to the ribotoxin family, whose best characterized member isa-sarcin (EC 3.1.27.10). The proteins of this group are cytotoxic ribonucleases that degrade a unique bond in ribosomal RNA impairing protein biosynthesis.

9p awards 06-04-2013 37 3   Download

TheX-raystructureofnativecellobiohydrolase IB(CBHIB) from the filamentous fungus Talaromyces emersonii,PDB 1Q9H,was solved to2.4 A ˚ bymolecular replacement. 1Q9H is a glycoprotein that consists of a large, single domain with dimensions of
60 A ˚ ·40 A ˚ ·50 A ˚ andanoverall b-sandwich structure, the characteristic fold of Family 7 glycosyl hydrolases (GH7). It is the first structure of a native glycoprotein and cellulase fromthis thermophilic eukaryote.

12p awards 05-04-2013 37 2   Download

The 3D-solution structure ofNeurospora crassaCu6 -metal-lothionein (NcMT) polypeptide backbone was determined using homonuclear, multidimensional 1 H-NMR spectros-copy. It represents a new metallothionein (MT) fold with a protein chain where the N-terminal half is left-handed and the C-terminal half right-handedly folded around a cop-per(I)-sulfur cluster. As seen with other MTs, the protein lacks definable secondary structural elements; however, the polypeptide fold is unique.

9p awards 05-04-2013 61 4   Download

Propionate is used to protect bread and animal feed from moulds. The mode of action of this short-chain fatty acid was studied usingAspergillus nidulansas a model organism. The filamentous fungus is able to grow slowly on propio-nate, which is oxidized to acetyl-CoA via propionyl-CoA, methylcitrate and pyruvate. Propionate inhibits growth of A. nidulanson glucose but not on acetate; the latter was shown to inhibit propionate oxidation.

15p awards 05-04-2013 46 3   Download

We describe a strategy for systematic amplification of chitin synthase genes (chs) in the filamentous ascomycetes plant-pathogenBotrytis cinereausing PCR with multiple degen-erate primers designed on specific and conserved sequence motifs. Eight distinctchsgeneswere isolated, namedBcchs I, II, IIIa, IIIb, IV, V, VIandVII. They probably constitute the entire chsmultigenic family of this fungus, as revealed by careful analysis of six euascomycetes genomes.

12p dell39 03-04-2013 38 4   Download