6/29/10
1
Seventh Meeting of the Lower Mekong Working Group
for Foot and Mouth Disease and Animal Movement
Management, Pakse, Lao PDR
6-7 November, 2008
FOOT AND MOUTH DISEASE,
VIETNAM/AAHL CARD PROJECT
C. Morrissy1, Ngo Thanh Long2, L. Wright1, Dong Manh Hoa2, Pham Phong Vu2,
Nguyen Thanh Phong2, Ly Hoai Vu2, I. Pritchard1, S. Riddell1, D. Schafer1, D. Eagles1,
W. Ha1, W. Goff1, J. Hammond3, S. Juzva1, M. Johnson1 and P. Daniels1.
1CSIRO Livestock Industries, Australian Animal Health Laboratory (AAHL),Geelong, Victoria, Australia.
2Regional Animal Health Office No. 6, Ho Chi Minh City (RAHO6-HCMC), South Vietnam.
3Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey, United Kingdom.
Project Objectives
Two Main Objectives:
• Improve Laboratory Capacity For Diagnosis Of FMD
― 
Serology, Sero-typing and Geno-tpying
•  ELISA, Cell culture, PCR & Sequencing
―  Quality Assurance
―  Improved Bio-safety & Workflow
―  Sample collection, Disease Investigation and Diagnosis
• Field Activities
― 
Application of Diagnostics
―  Serosurveillance & Epidemiology
―  Sample collection, Data collection, Disease Investigation and
Diagnosis
6/29/10
2
Geography of the
project
1. LANG SON
2. QUANG NINH
3. QUANG NAM
4. KONTUM
5. BINH PHUOC
6. TAY NINH
7. LONG AN
8. DONG THAP
9. AN GIANG
10. KIEN GIANG
Location of Provinces & Laboratories in Project
Project Outline
1. Setup pilot zones for effective vaccine application & surveillance
strategies:
•  10 Provinces
—  2 North, 2 Centre & 6 South
—  Provinces at borders with China, Laos & Cambodia
•  1 District in each Province
•  2 Communes in each District
•  1 Commune with 100% vaccination and 1 other chosen at
random from district
2. Serum collection from each province
• 120 cattle & 120 pig sera collected each round from each District
(Total 2400 each round)
• Aim is to look at effectiveness of vaccination and exposure to FMD virus
3. Samples for serotyping are collected routinely by DAH from
whole of Vietnam which include pilot zones
• Project to carry out serotyping ELISA, Virus Isolation and Genotyping
Breakdown of
Outbreaks
Pigs Cattle
Number 42 22
% 66 34
Breakdown of Outbreaks
Pigs Cattle Buffalo
Number 134 32 5
% 78 19 3
6/29/10
3
Breakdown of Outbreaks
Pigs Cattle
Number 42 1
% 98 2
Breakdown of Outbreaks
Pigs Cattle
Number 2 2
% 50 50
ELISA Serotyping Results Vietnam
Year Total
Samples
O A Asia 1 Negative /
Unsuitable
2005 251
216
* Sth Vietnam
207
180
17
17
13
11
12/2
6/2
2006 803
506
663
467
0
0
8
2
128/4
33/4
2007 224
123
177
115
1
1
8
0
7/0
7/0
Outcomes for the Surveillance
Sequencing/Genotyping 2006/2007
•  Serotype O: 3 topotypes
―  Cathay (33%), ME-SA (PanAsia) (6%)
& SEA (Myanmar 98) (41%)
―  Pigs: mainly Cathay & SEA
―  Cattle: mainly SEA
•  Serotype A: Thailand/Malaysia 97
―  Vaccine used for FMD Serotype A needed to change
from A 22 to A Malaysia 97 (also antigen for serology)
•  Serotype Asia 1: Jiangsu-China-2005 (Nth & Centre)
Myanmar 98 (Centre)
―  Confirmed 2 sources of Asia 1 virus into Vietnam
6/29/10
4
Outcomes for the Surveillance
Sero-surveillance
•  Vaccination efficacy
—  LP ELISA (O, A22, A Malaysia, Asia 1)
•  Prevalence of infection
—  AAHL 3ABC C-ELISA
•  Sero-typing using serology data was
possible
—  using LP ELISA titres from NS positive animals
Vaccination Coverage in Cattle
Results form Sero-surveillance
Vaccine Response Rate in Cattle
vaccinated with Serotype O
0%
20%
40%
60%
80%
100%
2006 2007 (1) 2007 (2) 2008
(6mths)
Commercial
Farm
Vaccination Response in Cattle (Based on LP Elisa type O results)
6/29/10
5
Vaccine Response Rate in Cattle
vaccinated with Serotype O
Year Number
vaccinated with
serotype O
Number LP -Elisa
(O) positive
% vaccinated that
were LP-Elisa (O)
positive
2006 265 78 29%
2007 (1) 638 306 48%
2007 (2) 630 352 56%
2008
6 mths post
vaccination
598 180 30%
Commercial Farm
(2008)
96 86 90%
Post Vaccination titres for Commercial Cattle
Total number of cattle in trial = 95
Vaccination Coverage in Pigs
0%
10%
20%
30%
40%
50%
60%
70%
80%
2006 2007 (1) 2007 (2) 2008
(6mths)
Commercial
Farm
Vaccination Response Rates in Pigs (Based on LP Elisa O results)
Vaccine Response Rate in Pigs
vaccinated with Serotype O