Effect of duration of cold pretreatment and plant growth regulators on callusing and green plant regeneration of Indica (Oryza sativa L.) rice

Improvement of indica rice lines is a challenging task through application of anther culture as they are recalcitrant to culture distinctive to japonica rices. Current investigation aimed to verify the effect of cold pretreatment and phytohormones on the anther culture response of 6 crosses. Cold pretreatment for 7-10 days at 100C was found to have profound effect on callus induction frequency irrespective of the media employed and the prolonged treatment over the optimum showed to be inhibitory...