Kspd= 157lmand katp= 215lm.

Glutathionylspermidine is an intermediate formed in the biosynthesis of trypanothione, an essential metabolite in defence against chemical and oxi-dative stress in the Kinetoplastida. The kinetic mechanism for glutathionyl-spermidine synthetase (EC 6.3.1.8) from Crithidia fasciculata (CfGspS) obeys a rapid equilibrium random ter-ter model with kinetic constants KGSH= 609lm, KSpd= 157lmand KATP= 215lm.

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