Oxidized coenzymes

The effect of CoA on the characteristic light decay of the firefly luciferase catalysed bioluminescence reaction was studied. At least part of the light decay is due to the luciferase catalysed formation of dehydroluciferyl-adenylate (L-AMP), a by-product that results from oxidation of luciferyl-adenylate (LH2-AMP), and is a powerful inhibitor of the bioluminescence reaction (IC50¼6nm).

11p fptmusic 12-04-2013 39 4   Download

The roles of His181, His184 and Tyr186 in PETN reductase have been examined by mutagenesis, spectroscopic and stopped-flow kinetics, and by determination of crystallographic structures for the Y186F PETN reductase and reduced wild-type enzyme—progesterone complex. Residues His181 and His184 are important in the binding of coenzyme, steroids, nitro-aromatic ligands and the substrate 2-cyclohexen-1-one.

12p fptmusic 12-04-2013 40 3   Download

The Baeyer–Villiger monooxygenase (BVMO), 4-hydroxy-acetophenone monooxygenase (HAPMO), uses NADPH andO2 to oxidize a variety of aromatic ketones and sulfides. The FAD-containing enzyme has a 700-fold preference for NADPH over NADH. Sequence alignment with other BVMOs, which are all known to be selective for NADPH, revealed three conservedbasic residues,whichcouldaccount for the observed coenzyme specificity. The corresponding residues in HAPMO (Arg339, Lys439 and Arg440) were mutated and the properties of the purified mutant enzymes were studied. ...

10p dell39 03-04-2013 28 2   Download

The functional activities of proteins are closely related to their molecular structure and understanding their structure–function relationships remains one of the intriguing problems of molecular biology. We investigated struc-tural changes in 17b-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus(17b-HSDcl) induced by pH, temperature, salt, urea, guanidine hydrochloride, and coenzyme NADPH binding.

11p inspiron33 25-03-2013 41 4   Download

The di-iron flavoprotein F420H2 oxidase found in methanogenic Archaea catalyzes the four-electron reduction of O2to 2H2O with 2 mol of reduced coenzyme F420(7,8-dimethyl-8-hydroxy-5-deazariboflavin). We report here on crystal structures of the homotetrameric F420H2 oxidase fromMethan-othermobacter marburgensis at resolutions of 2.25 A ˚ , 2.25 A ˚ and 1.7 A ˚ , respectively, from which an active reduced state, an inactive oxidized state and an active oxidized state could be extracted.

12p galaxyss3 21-03-2013 45 4   Download

The biosynthesis of lovastatin inAspergillus terreusrequires two mega-synthases. The lovastatin nonaketide synthase, LovB, synthesizes the inter-mediate dihydromonacolin L using nine malonyl-coenzyme A molecules, and is a reducing, iterative type I polyketide synthase.

11p galaxyss3 19-03-2013 33 5   Download

Formate dehydrogenase from Candida boidinii(CboFDH) catalyses the oxidation of formate anion to carbon dioxide with concomitant reduction of NAD + to NADH. CboFDH is highly specific to NAD + and virtually fails to catalyze the reaction with NADP + . Based on structural informa-tion for CboFDH, the loop region betweenb-sheet 7 and a-helix 10 in the dinucleotide-binding fold was predicted as a principal determinant of coen-zyme specificity.

11p galaxyss3 07-03-2013 28 3   Download

Glutamate dehydrogenase (EC 1.4.1.2–4) fromPeptostreptococcus asacch-arolyticushas a strong preference for NADH over NADPH as a coenzyme, over 1000-fold in terms ofkcat ⁄Kmvalues. Sequence alignments across the wider family of NAD(P)-dependent dehydrogenases might suggest that this preference is mainly due to a negatively charged glutamate at position 243 (E243) in the adenine ribose-binding pocket.

8p media19 04-03-2013 39 3   Download

Elucidating the origin of substrate and coenzyme specificity has been the focus of much work relating to enzyme engineering. Many enzymes exhibit tight specificity for particular substrates despite a close structural relation-ship to other nonreactive compounds.

10p vinaphone15 25-02-2013 37 3   Download

The ubiquinone coenzyme Q (CoQ) is synthesized in mitochondria with a large, hydrophobic isoprenoid side chain. It functions in mitochondrial res-piration as well as protecting membranes from oxidative damage. Yeast that cannot synthesize CoQ (DCoQ) are viable, but cannot grow on nonfer-mentable carbon sources, unless supplied with ubiquinone.

16p mobifone23 21-01-2013 24 4   Download

Clostridial glutamate dehydrogenase mutants, designed to accommodate the 2¢-phosphate of disfavoured NADPH, showed the expected large speci-ficity shifts with NAD(P)H. Puzzlingly, similar assays with oxidized cofac-tors initially revealed little improvement with NADP + , although rates with NAD + were markedly diminished.

9p cosis54 05-01-2013 49 4   Download

Early metabolic steps, including glycolysis and the activity of the pyruvate dehydrogenase complex, yield a two-carbon fragment called an acetyl group, which is linked to a large cofactor known as coenzyme A (or CoA). It is during the citric acid cycle that acetyl-CoA is oxidized to the waste product, carbon dioxide, along with the reduction of the cofactors NAD+ and ubiquinone

94p zingzing09 21-10-2012 58 9   Download

Đây là bài giảng của thầy Đỗ Hiếu Liêm, trường ĐH Nông Lâm TP.HCM. Chuỗi hô hấp mô bào bao gồm các phản ứng oxid hoá khử liên tục trên các cơ chất (sản phẫm biến dưỡngtrung gian - Chu trình Krebs và β oxid hoá acid béo). Tổ chức: hệthống enzyme CHH với các coenzyme tổchức thành từng tổ hợp và sắp xếp theo thứ tự tăng dầnđiện thế oxid hoá khử,bắđầu NAD+ dehydrogenase có điện thế âm và cuối cùng là cytochrome oxidase có điện thế dương....

32p h08112107 22-08-2010 326 134   Download