Reaction kinetic method

This study aims at better understanding the chemical and physico-chemical phenomena of uranium dioxide dissolution reactions in nitric acid media in the Purex process, which separates the reusable materials and the final wastes of the spent nuclear fuels.

13p christabelhuynh 29-05-2020 13 1   Download

Microcalorimetry and UV-vis spectroscopy were used to conduct thermodynamic and kinetic investigations of the scission of calf thymus DNA catalyzed by bleomycin A5 (BLM-A5) in the presence of ferrous ion and oxygen. The molar reaction enthalpy for the cleavage, the Michaelis– Menten constant for calf thymus DNA and the turnover number of BLM-A5 were calculated by a novel thermokinetic method for an enzyme-catalyzed reaction to be )577 ± 19 kJÆmol)1, 20.4 ± 3.8 lM and 2.28 ± 0.49 · 10)2 s)1, respectively, at 37.0 °C. This DNA cleavage was a largely exothermic reaction....

9p system191 01-06-2013 31 4   Download

The dual nucleotide cofactor-specific enzyme, 3a-hydroxy-steroid dehydrogenase (3a-HSD)from Pseudomonassp. B-0831, is a member of the short-chain dehydrogenase/ reductase (SDR)superfamily. Transient-phase kinetic stud-ies using the fluorescence stopped-flow method were con-ducted with 3a-HSD to characterize the nucleotide binding mechanism. The binding of oxidized nucleotides, NAD + , NADP + and nicotinic acid adenine dinucleotide (NAAD + ), agreed well with a one-step mechanism, while that of reduced nucleotide, NADH, showed a two-step mechanism. ...

7p dell39 03-04-2013 39 4   Download

Kinetic modelling of complex metabolic networks – a central goal of com-putational systems biology – is currently hampered by the lack of reliable rate equations for the majority of the underlying biochemical reactions and membrane transporters.

15p vinaphone15 27-02-2013 45 2   Download

The aim of the study was to investigate the crystallization kinetics and solidification behaviour of Fe60Co8Mo5Zr10W2B15 bulk glass forming alloy. The solidification behaviour in near-equilibrium and non-equilibrium cooling conditions was studied. The eutectic and peritectic reactions were found to exist in the solidification sequence of the alloy. The bulk metallic glass formation was achieved by using two methods: quenching from the liquid state and quenching from the semi-state.

140p dontetvui 21-01-2013 82 12   Download