A comparative study on colchicine application methods in obtaining doubled haploids of tobacco (Nicotiana tabacum L.)

In order to obtain androgenic doubled haploids of tobacco (Nicotiana tabacum cv. Karaba¤lar 6265) colchicine was applied at 3 different stages of anther culture. The main advantage of this method is that treated plantlets can be transplanted directly into pots in order to grow androgenic plants.