Amylase and biosurfactant production from Bacillus species

Most Bacillus species have ability to produce secondary metabolites such as amylases and biosurfactants. This study was carried out to determine the amylase and boisurfactant production potentials of some Bacillus species obtained from agricultural soil. Soil samples were collected from agricultural research farm of Adekunle Ajasin University, Akungba-Akoko, Ondo State, Nigeria. All isolates were identified based on the morphological and biochemical characteristics using Bergey’s manual of determinative bacteriology. Amylase-producing Bacillus species were selected and screened on soluble starch agar while biosurfactant activity was determined by three methods; blood haemolysis, oil spreading, and drop-collapse tests.