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Báo cáo khoa học: Expression in yeast of a novel phospholipase A1 cDNA from Arabidopsis thaliana

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:13

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During a search for cDNAs encoding plant sterol acyl-transferases, we isolated four full-length cDNAs fromAra-bidopsis thaliana that encode proteins with substantial identity with animal lecithin : cholesterol acyltransferases (LCATs). The expressionof one of these cDNAs,AtLCAT3 (At3g03310), invarious yeast strains resulted in the doubling of the triacylglycerol content. Furthermore, a complete lipid analysis of the transformed wild-type yeast showed that its phospholipid content was lower than that of the control (void plasmid-transformed) yeast whereas lysophospho-lipids and free fatty acids increased....

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Nội dung Text: Báo cáo khoa học: Expression in yeast of a novel phospholipase A1 cDNA from Arabidopsis thaliana

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