Báo cáo khoa học: Expression, purification and catalytic activity of Lupinus luteus asparagine b-amidohydrolase and its Escherichia coli homolog
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We describe the expression, purification, and biochemical characterization of two homologous enzymes, with amido-hydrolase activities, of plant (Lupinus luteuspotassium-independent asparaginase, LlA) and bacterial (Escherichia coli, ybiK/spt/iaaA gene product, EcAIII) origin. Both enzymes were expressed inE. colicells, with (LlA) or with-out (EcAIII) aHis-tag sequence. The proteins were purified, yielding6 or 30 mgÆL )1 of culture, respectively.
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