Báo cáo khoa học: Tritium planigraphy comparative structural study of tobacco mosaic virus and its mutant with altered host specificity

Spatial organizationofwild-type (strainU1) tobaccomosaic virus (TMV) and of the temperature-sensitive TMV ts21–66 mutant was compared by tritium planigraphy. The ts21–66 mutant contains two substitutions in the coat protein (Ile21fiThr and Asp66fiGly) and, in contrast withU1, induces a hypersensitive response (formation of necroses) on the leaves of plants bearing a host resistance geneN¢(for example Nicotiana sylvestris); TMV U1 induces systemic infection (mosaic) on the leaves of such plants. Tritium dis-tribution along the coat protein (CP) polypeptide chain was determined after labelling of bothisolated CP preparations and intact virions. ...