zunia.vn

Tuyển sinh 2024 dành cho Gen-Z

zunia.vn

» Luận Văn - Báo Cáo

» Báo cáo khoa học

Báo cáo lâm nghiệp: "Influence of the form of nitrogen nutrition reductase activity in young black locus"

Chia sẻ: Nguyễn Minh Thắng | Ngày: | Loại File: PDF | Số trang:4

Báo xấu

38
lượt xem 4
download

Download Vui lòng tải xuống để xem tài liệu đầy đủ

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: Influence of the form of nitrogen nutrition reductase activity in young black locust...

Chủ đề:

Bình luận(0) Đăng nhập để gửi bình luận!

Lưu

Nội dung Text: Báo cáo lâm nghiệp: "Influence of the form of nitrogen nutrition reductase activity in young black locus"

Influence of the form of nitrogen nutrition foliar nitrate on reductase activity in young black locust ( Robinia pseudoacacia L.) F. Boutekrabt G. Pizelle Laboratoire de BP 239, 54506 Physiologie V6g6tale et Foreshore, Facult6 des Sciences, Vand&oelig;uvre-Iès-Nancy Cedex, Francqe Nitrogenase (N activity was measured Introduction ase) 2 intact plantlets by the acetylene reduction on method. Nitrate reductase (NR) activity was measured in vivo on leaf disks (diameter: 12 Robinia pseudoacacia L. fixes dinitrogen mm). Two disks were vacuum infiltrated in 3 ml in symbiosis with a specific rhizobium and, of 0.1 M NaK phosphate buffer, pH 7.5, contain- as a nitrogen-fixing tree, gives a litter with ing 0.05 M KN0 and Triton X-100 (0.1 %, v/v). 3 a low C/N (Moiroud and Capellano, 1981 ), After incubation for 1 h at 30°C in the dark, was determined spectrometrically at 540 NOi which favors nitrification (Montagnini et nm m the incubation medium. al., 1986). In the field, black locust can therefore both symbiotic and nitrate nitrogen. use Data on the nitrate reduction in R. pseu- Results doacacia are still very limited (Aznadi, 1984). The present work examines the Presence of a constitutive teaf NR activity effects of the symbiotic and nitrate nitro- affected by fhe N ase activity 2 gen nutritions on the leaf nitrate reductase activity and the distribution of this enzyme activity in young black locusts. The leaves of nitrogen-deficient plants had a notable NR activity considered as constitutive, since it was not induced by nitrate (Fig. 1This enzyme activity Materials and Methods decreased to a minimum value (!1 nmol N° DW!h-1) when the N in- 1 "’mg- 2 ase 2 black locusts inoculated with a suspen- Young creased after nodulation. When symbiotic sion of crushed nodules were grown on a nitro- nitrogen nutrition was eliminated by ex- gen-free nutrient solution in a growth chamber. cision of root nodules, a notable NR activi- Nitrate nutrition of the nodulated plants was ty was progressively recovered in the assured by the addition of 4 or 10 mM NaN0 3 leaves (results not shown). to the nitrogen-free solution.
NR activity Effect of nitrate of leaf NR activity of new leaf expanded, the decreased in the previous leaf and the nodulafed plants highest enzyme activity was found again in the new leaf (Fig. 2). Administration of 4 mM NaN0 to 11 mo 3 When the nitrate supply was withdrawn, old nodulated plants did not increase leaf the enzyme activity recovered its minimum NR activity, whereas the 10 mM NaN0 3 value after 2 d (Fig. 3). dose induced high enzyme activity (Fig. 2). After 72 h of induction, the highest NR activity was found in the apical fully expanded leaf and corresponded with the highest nitrate content (Table I). When a Conclusion Notable NR activity (6-9 nmol 1 N02-mg- ) 1 DW-h- measured in vivo in the was leaves of young nitrogen-deficient R. pseudoacacia; its role is unknown, but its decrease, concomitant with the advent of the N activity, indicates a relationship ase 2 between both enzyme activities. The low NR activity (!1 nmol N0 DW!h-!) of 1 -mg- 2 nodulated plants could be greatly increas- ed by nitrate supplied via the roots. This inducible NR activity was consistently
highest in the younger expanded leaves nutrition azot6e du robinier (Robinia pseudoa- cacia L.). Thesis 3rd Cycle, Universit6 de that showed the highest nitrate content. Nancy I, France Studies are in progress to compare these Moiroud A. & Capellano A. (1981) Fixation results with those obtained on leaves from d’azote chez les espbces ligneuses symbio- field-grown R. pseudoacacia. tiques. II. Reprise de I’activit6 fixatrice (rbduc- tion de C chez Robinia pseudoacacia L. au ) H 2 printemps. Bull. Soc. Bot. 128, 239-247 Haines B., Boring L. & Swang W. Montagnini F., References (1986) Nitrification potentials in early succes- sional black locust and in mixed hardwood Aznadi A. (1984) Etude de l’influence de forest stands in the southern Appalachians. herbicides la croissance et la quelques Biogeochemistry 2, 197-210 0 sur

CÓ THỂ BẠN MUỐN DOWNLOAD

LV.01: Bộ Luận Văn Thạc Sĩ Quản Trị Kinh Doanh MBA

165 tài liệu

2069 lượt tải

THÔNG TIN

TRỢ GIÚP

HỖ TRỢ KHÁCH HÀNG

Theo dõi chúng tôi

Chịu trách nhiệm nội dung:

Nguyễn Công Hà - Giám đốc Công ty TNHH TÀI LIỆU TRỰC TUYẾN VI NA

LIÊN HỆ

Địa chỉ: P402, 54A Nơ Trang Long, Phường 14, Q.Bình Thạnh, TP.HCM

Hotline: 093 303 0098

Email: support@tailieu.vn

Giấy phép Mạng Xã Hội số: 670/GP-BTTTT cấp ngày 30/11/2015 Copyright © 2022-2032 TaiLieu.VN. All rights reserved.