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Comparison of solid-phase extraction methods for efficient purification of phosphopeptides with low sample amounts

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Efficient phosphoproteomic analysis of small amounts of biological samples (e.g. tissue biopsies) requires carefully selected enrichment and purification steps prior to the nanoflow HPLC-MS/MS analysis. Solidphase extraction (SPE) is one of the most commonly used approaches for sample preparation.

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Nội dung Text: Comparison of solid-phase extraction methods for efficient purification of phosphopeptides with low sample amounts

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