Isolation, identification and optimization of alkaline amylase production from bacillus cereus using Agro-industrial wastes

Amylase with maximum activity at alkaline pH prefers most industrial applications. The present study investigate amylase production and optimization by bacterial strain isolated and identified from rice mill waste dumped soil. The potent isolate KR9 was identified based on biochemical characteristics and 16S rRNA gene analysis confirmed that this isolate belonged to the genus Bacillus and it was most closely related to B. cereus (99 % similarity). The selected isolate B. cereus KR9 was optimized for pH, growth period and carbon and nitrogen sources.