Keap1/NRF2-independent antioxidative activity of Phyllanthus amarus extract in zebrafish

The Keap1 protein (Kelch-like ECH-related protein 1) and the Nrf2 transcription factor (NF-E2- related factor 2) are important systems for maintaining homeostasis, redox, and metabolism. Based on the Keap1/Nrf2 pathway, the antioxidative mechanism of P. amarus extract (PAE) was predicted. In this paper, we evaluated the protective effects of PAE on the oxidative toxicity induced by sodium arsenite (NaAsO2) and hydrogen peroxide (H2O2) in zebrafish larvae.