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Optimization of L-asparaginase production from Escherichia coli using response surface methodology
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Response surface methodology (RSM) is an efficient strategic experimental tool by which the optimal conditions of a multivariable system can be determined. In the present study, strain recombinant E. coli BL21(DE3) harboring gene L-asparaginase was optimized expression condition using design of experiments and response surface methodology to enhance the production of the active form of recombinant Lasparaginase. The biological activity of recombinant L-asparaginase was also tested on human blood cancer cell line.
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