Production of amylase from Bacillus thuringiensis J2 using apple pomace as substrate in solid state fermentation

Amylase producing thermophilic bacterial strain was isolated from Jeori hot water spring, Rampur Bushahr of Shimla district of Himachal Pradesh, India and identified as Bacillus thuringiensis J2 using 16S rRNA gene sequencing and deposited in NCBI gene bank vide accession number |KY990713|. Medium components and process parameters were optimized with respect to substrate concentration, carbon sources, nitrogen sources, incubation period, pH and temperature for an enhanced amylase production.