Screening populations for copy number variation using genotyping-by-sequencing: a proof of concept using soybean fast neutron mutants

The effective use of mutant populations for reverse genetic screens relies on the population-wide characterization of the induced mutations. Genome- and population-wide characterization of the mutations found in fast neutron populations has been hindered, however, by the wide range of mutations generated and the lack of affordable technologies to detect DNA sequence changes.