Selection of genotype and mutagens in M1 generation for establishment of mutation breeding programme in mungbean [Vigna radiata (L.) Wilczek]

An experiment was conducted using seeds of two mungbean verities viz., WGG-42 and LGG-460 treated with various doses/ concentrations of gamma rays (200, 300, 400, 500 and 600 Gy) and ethyl methane sulphonate (0.2%, 0.3%, 0.4%, 0.5% and 0.6%) and sodium azide (1 mM, 2 mM and 3 mM) to find the LD50 dose by using probit analysis in M1 generation. LD50 dose is important to understand the sensitivity of various genotypes to the critical dose of mutagens creating 50 percent mortality.