Studies of ploidy assessment in some synthetic hybrids of banana (Musa spp.)

Banana (Musa spp.) constitute a hybrid-polyploid complex and are classified according to different genome compositions such as AA, BB, AB, AAA, AAB, ABB, AAAA, ABBB, AAAB, AABB and AAABB. Knowledge of ploidy and exact genome compositions of the parental material is essential for Musa breeding. Flow cytometric analysis of nuclear DNA content was used to estimate ploidy levels. Twenty four Banana hybrids under phase-I and nineteen hybrids under phase-II evaluated and was done by flow cytometry analysis which enables rapid and precise measurements on whole cells, isolated nuclei or chromosomes in a monodisperse suspension.