Study on determination of nifedipine in dog plasma by ultra performance liquid chromatography with tandem mass spectrometric detection

To validate the determination method of nifedipine (NIF) in dog plasma by ultra performance liquid chromatography with tandem mass spectrometric detection (UPLC-MS/MS). The evaluation of the specificity, selectivity, lower limit of quantitation, accuracy, precision, calibration curve and the linear range, recovery, carry-over, matrix effect, stability of NIF in plasma following the guidance of US-FDA.