Usability of reference-free transcriptome assemblies for detection of differential expression: A case study on Aethionema arabicum dimorphic seeds

RNA-sequencing analysis is increasingly utilized to study gene expression in non-model organisms without sequenced genomes. Aethionema arabicum (Brassicaceae) exhibits seed dimorphism as a bet-hedging strategy – producing both a less dormant mucilaginous (M+ ) seed morph and a more dormant non-mucilaginous (NM) seed morph.