16S ribosomal RNA gene

Anaplasmosis, an animal disease caused by rickettsial bacteria in the genus Anaplasma, is of considerable economic importance in livestock animals in many countries worldwide. The objectives of this study were to determine the identity, prevalence, and geographic distribution of Ehrlichia and Anaplasma in naturally infected water buffalo in Thailand using PCR amplification and sequencing of the 16S ribosomal RNA and heat shock protein groEL genes.

12p vigandhi 23-02-2022 13 1   Download

Gut microbes play an important role in the growth and development of fish. The Tibetan Plateau fish Glyptosternum maculatum is a unique species of sisorid catfish living in the river up to 4200m altitude.

9p vidarwin 22-02-2022 8 1   Download

Inferring phylogenetic trees for newly recovered genomes from metagenomic samples is very useful in determining the identities of uncultivated microorganisms. Even though 16S ribosomal RNA small subunit genes have been established as “gold standard” markers for inferring phylogenetic trees, they usually cannot be assembled very well in metagenomes due to shared regions among 16S genes. Using single-copy marker genes to build genome trees has become increasingly popular for uncultivated species.

10p vilarryellison 29-10-2021 9 1   Download

Tách chiết và giải trình tự thành công gene 16S-rRNA của ba mẫu tôm thẻ chân trắng (Penaeus vannamei) Việt Nam. Hai mươi sáu loài thuộc họ tôm he (Penaeidae) được phân thành 8 nhóm dựa trên gene chỉ thị 16S-rRNA. Nhóm I có 5 loài, nhóm II có 4 loài, nhóm III có 5 loài, nhóm IV có 6 loài, nhóm V có 1 loài, nhóm VI có 1 loài, nhóm VII có 3 loài và nhóm VIII có 1 loài. Khoảng cách di truyền của nhóm III và nhóm V là gần nhất 0,272.

7p vining2711 09-08-2021 24 1   Download

Studies on the airway microbiome have been performed using a wide range of laboratory protocols for high-throughput sequencing of the bacterial 16S ribosomal RNA (16S rRNA) gene. We sought to determine the impact of number of polymerase chain reaction (PCR) steps (1- or 2- steps) and choice of target marker gene region (V3 V4 and V4) on the presentation of the upper and lower airway microbiome.

15p vilichoo2711 25-06-2021 14 1   Download

Aspiration pneumonia has been a growing interest in an aging population. Anaerobes are important pathogens, however, the etiology of aspiration pneumonia is not fully understood. In addition, the relationship between the patient clinical characteristics and the causative pathogens in pneumonia patients with aspiration risk factors are unclear.

12p vimaine2711 26-03-2021 10 1   Download

Culture-independent phylogenetic analysis of 16S ribosomal RNA (rRNA) gene sequences has emerged as an incisive method of profiling bacteria present in a specimen. Currently, multiple techniques are available to enumerate the abundance of bacterial taxa in specimens, including the Sanger sequencing, the ‘next generation’ pyrosequencing, microarrays, quantitative PCR, and the rapidly emerging, third generation sequencing, and fourth generation sequencing methods.

11p viwyoming2711 16-12-2020 14 0   Download

Long et al. (BMC Bioinformatics 2014, 15(1):278) describe a “discrepancy” in using UniFrac to assess statistical significance of community differences. Specifically, they find that weighted UniFrac results differ between input trees where (a) replicate sequences each have their own tip, or (b) all replicates are assigned to one tip with an associated count.

3p vikentucky2711 24-11-2020 17 2   Download

Prokaryotic 16S ribosomal RNA (rRNA) sequences are widely used in environmental microbiology and molecular evolution as reliable markers for the taxonomic classification and phylogenetic analysis of microbes.

8p vioklahoma2711 19-11-2020 11 1   Download

Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this approach strongly depends on the choice of primer pairs and, in particular, on the balance between efficiency, specificity and sensitivity in the amplification of the different bacterial 16S sequences contained in a sample.

10p viconnecticut2711 28-10-2020 11 0   Download

The aim of this study was to support for finding novel mechanisms that render bacteria resistant to the ribosome targeting antibiotics, we screen for antibiotic resistant 16S and 23S ribosomal RNAs (rRNAs) in multidrug resistant Salmonella serovars isolated from raw retail meats isolated from Hanoi, Vietnam. Bioinformatic analysis identified 193 unknown novel mutations (64 mutations in 16S rRNA and 129 mutations in 23S rRNA genes).

8p nguyenxuankha_bevandan 14-08-2020 19 3   Download

Leptospirosis, a zoonosis caused by Leptospira, is recognized as an emergent infectious disease. In currently, the lack of adequate diagnostic tools, vaccines are an attractive intervention strategy. In this experiment, a 550 bp fragment of large ribosomal RNA gene (16S rRNA) was sequenced and constructed phylogenetic tree from a panel of six Vietnamese pathogenic strains of Leptospira spirochetes (e.g., Pomona, Canicola, Mitis, Ictero haemohagiae, Bataviae, and Grippotyphosa).

12p nguyenxuankha_bevandan 14-08-2020 10 1   Download

The 16S rRNA gene is composed of highly conserved, specie-specific sequences between different species of bacteria and the application of restriction endonucleases on the amplified 16S rRNA gene is a novel diagnostic tool in molecular characterization of bacterial isolates.

10p nguaconbaynhay6 23-06-2020 11 0   Download

Sự phát triển của ngành công nghệ thông tin trong những thập kỷ qua đã góp phần cải thiện chất lƣợng cuộc sống con ngƣời. Máy tính đã có mặt trong hầu hết các ngành khoa học, hỗ trợ con ngƣời giải quyết những công việc khó khăn và nhiều thời gian. Công nghệ sinh học đƣợc xem là ngành khoa học mũi nhọn của thế kỷ XXI. Với sự ra đời của kỹ thuật giải trình tự, nhiều bộ gene của sinh vật đã đƣợc giải mã, tạo ra một lƣợng dữ liệu sinh học khổng lồ. Điều này...

83p canhchuon_1 21-06-2013 98 22   Download