Agar plate method

This study aims to evaluate the microbial contamination level and identify bacterial colonization of the operating room in the hospital. Methods: Three sampling procedures were used in this study, which includes swabbing, air sampler and contact agar plate. Collected samples from 8 ORs were transported and microbiologically processed using standard procedures.

6p vischultz 17-10-2023 3 2   Download

Mycoparasitism by Trichoderma sp. is one of the important modes of action exhibited by biocontrol agents against host fungi including plant pathogenic ones. Its study in-vitro constitutes considerable importance while studying bio-efficacy of an antagonist against a fungal pathogen. At present there is no standard, rapid, easy and reliable method to study mycelial interaction between a possible mycoparasitic fungi and host.

5p angicungduoc8 07-11-2020 6 1   Download

The experiments were conducted at Department of Plant Pathology, S.K.N. College of Agriculture, Jobner (Rajasthan). Five samples of blonde psyllium seeds were collected from Barmer, Nagaur, Pali, Jodhpur and Jalore districts of Rajasthan and tested for seed borne fungi. Both Blotter and Agar Plate methods were used to detect the maximum number of mcontrol and external seed borne fungi. A total of 5 species of fungi were identified using the two selective methods. The pathogencity of A. alternata was studied on psyllium seeds and seedlings.

6p nguaconbaynhay8 13-10-2020 14 2   Download

In light of the same, the objectives of this study include to isolate various Staphylococcus species from clinical specimens. And also to compare three phenotypic methods for detection of biofilm formation among the isolated Staphylococcus species, namely, Tube adherence method, Tissue culture plate method and Congo red agar method.

9p nguaconbaynhay7 15-08-2020 13 1   Download

The aim of this study was to detect biofilm formation by study isolates of Pseudomonas aeruginosa qualitatively and quantitatively. Ten isolates were taken from diseased animal in veterinary clinics of RAJUVAS Bikaner. Qualitative biofilm formation assays (Pellicle formation assay and Congo red agar method) and quantitative assay by Microtitre plate assay used for biofilm production.

6p nguaconbaynhay7 15-08-2020 13 1   Download

In the present study attempts have been made on the isolation and identification of fungi in order to study the mycoflora responsible to reduce the seed quality, longevity and vigor in the pulse of Hyacinth bean. Lablab purpureus L. is an annual plant that grows in poorly - drained soils belongs to the family Fabaceae. It contains high amount of protein, vitamins and minerals. Fungal cultures were isolated by using blotter and agar plate methods.22 fungal species and 14 genera were isolated.

5p nguaconbaynhay6 24-06-2020 13 0   Download

The objective of the present study was to identify protease producing bacteria isolated from food processing industries. Isolation of the organism was performed by serial dilution agar plate technique and initial screening of protease production was done using gelatine agar plates by flooded the plates with mercuric chloride solution (HgCl2-15g and 20 ml of 6.0 N HCl made up to 100 ml with distilled water). A total of 5 isolates were selected based on zone diameter in gelatine clear zone method and named as TKMFT8, TKMFT22, TKMFT25, TKMFT39 and TKMFT53.

12p nguaconbaynhay6 24-06-2020 14 1   Download

Objective of the research was to validate the potential of Syzygium aromaticum and Kaempheria galanga against biofilm formation and plasmid borne drug resistance exhibited by Pseudomonas spp. Antimicrobial property of S.aromaticum and K.galanga on P.aeruginosa culture was assessed using the agar well diffusion method. Crystal violet assay was employed to determine the percentage inhibition of P.aeruginosa biofilm by the extracts following generation of biofilm on multiwell plates.

7p nguaconbaynhay6 23-06-2020 30 0   Download

This study aimed to determine bacteriological quality of indoor air in neonatal wards at tertiary hospitals and assess antibiotic resistance patterns of isolated bacteria. Passive air sampling was performed to appraise bacteriological indoor air quality employing the gravity settle plate method to monitor air quality in the morning and afternoon. Each procedure was performed in duplicate on Nutrient Agar plates for 30 and 60 minutes. A total of 75 bacteria were isolated and subjected to antibiotic sensitivity tests using the Kirby-Bauer’s disk diffusion method.

9p nguaconbaynhay6 23-06-2020 8 0   Download

Horse gram (Macrotyloma uniflorum), belongs to the family Fabaceae is one of the minor or lesser known neglected legume mainly cultivated due to high levels of protein. It is considered as poor man’s pulse. Horse gram has an excellent nutritional, therapeutic and medicinal properties. Field variety of horse gram seeds were collected from the Indurthi village, Kharimnagar district, Telangana, India. The legume seed borne fungi was screened by employing blotter paper method, agar plate method and dilution plate method.

6p nguaconbaynhay6 23-06-2020 12 1   Download

Chickpea wilt caused by F. oxysporum f. sp. ciceri is one of the most important seed and soil-borne disease in India. The study on association of the pathogen with chickpea seeds showed that the viable mycelium fragments, micro and macro-conidia of the pathogen were present on the seed surface of all susceptible and two resistant varieties/ germplasms in seed washing test. The pathogen was recovered from the untreated and sodium hypochlorite treated seeds of all susceptible varieties/ germplasms in the range of 10.67– 18.67 and 5.33–11.

10p cothumenhmong5 17-05-2020 11 2   Download

Soil microorganisms such as bacteria and fungi play an important role in soil fertility and promoting the plant growth. Sixty soil samples are collected of different agricultural crop fields such as groundnut field, sugarcane field, Plantain field, turmeric field, tapioca field and wasteland with Parthenium field in and around Thiruvannamalai, Tamil Nadu were investigated soil moisture content by oven drying method and diversity of fungi by using serial dilution plating method with standard Potato Dextrose Agar (PDA) for qualitative and quantitative pattern.

15p trinhthamhodang5 16-05-2020 12 1   Download

Biofilm is the population of bacterial cells growing on the biotic and abiotic surfaces embedded in a matrix of extracellular polysaccharide, which facilitate the adherence of microorganism. This study is aimed to compare and evaluate different Methods for detection of Biofilm formation in Staphylococcus aureus isolates. For comparison of different methods of Biofilm production, 100 isolates of Staphylococcus aureus (50 MRSA and 50 MSSA) were taken and screened by tissue culture plate method, tube method in different condition and Congo Red Agar (CRA) plate method...

8p angicungduoc4 04-05-2020 25 0   Download

Keeping in view the immense importance of seed the present investigation was carried out to detect 14 seed borne mycoflora of maize variety Vivek 27 by using Agar plate method and Blotter paper method. Per cent maximum relative density, frequency and abundance values recorded in Aspergillus niger...

12p kethamoi4 18-04-2020 12 1   Download

Health status of okra seed samples collected from six different location (Raipur, Kawardha, Bilaspur, Ambikapur, Jashpur and Dhamtari) of Chhattisgarh was studied. By incubation methods (standard blotter, agar plate and 2, 4-D), in total 14 mycoflora (Alternaria sp., Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Chaetomium sp., Colletotrichum sp., Curvularia sp., Fusarium sp., Memnoniella sp., Nigrospora sp., Penicillium sp., Rhizopus sp., Sterile mycelium, Trichoderma sp.) were detected with varying frequencies in different samples and methods.

8p nguaconbaynhay4 22-03-2020 14 0   Download

Streptococcus pyogenes was isolated from the throat swabs of infected individual and swabbed on the blood agar and sodium azide crystal violet blood agar plates. Isolated organism was grown on the semi-synthetic medium for enrichment and extraction of M protein. The extracted M protein was further purified. The purified M protein was estimated by techniques like Lowry’s method of protein estimation. The M protein was subjected to confirmation techniques and SDS-PAGE analysis.

14p caygaocaolon3 09-03-2020 7 0   Download

The treatment of methicillin resistant Staphylococcus aureus (MRSA) infection is challenging because of their high resistance to different antibiotics. The objectives of this study were to detect the prevelance of S. aureus and MRSA carriage in ICU patients in Al Quwayiyah General Hospital and to evaluate the performance of MRSA-Select agar media for rapid screening of MRSA Furthermore, to describe the antibiotic susceptibility patterns of the S. aureus and MRSA isolates. During the period from January to July 2018, a total of 278 patients admitted to the ICUs were screened for MRSA.

11p chauchaungayxua3 07-02-2020 16 0   Download

Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of hospitalacquired infections in many countries. Mainly healthcare workers (HCWs) are at risk for Methicillin-resistant Staphylococcus aureus carriage. A total of 100 healthcare workers (HCWs) were screened for carriage of MRSA. Nasal swabs collected from both anterior nares were transported to microbiology laboratory and inoculated on Mannitol salt agar plates. The plates were then incubated aerobically at 37o C for 24 hours and S. aureus was identified. The standard strains of S.

6p chauchaungayxua3 07-02-2020 16 0   Download

Corneal blindness is responsible for 1.5 to 2 million new cases of monocular blindness every year in which ocular trauma and corneal ulceration are significant contributors. Corneal infections of fungal etiology are common in India (19-45%). Aspergillus (16– 53%) and Fusarium (10–45%) species are the common pathogens. Purpose: 1.To Identify and isolate the various fungi causing fungal keratitis. 2. To know the usefulness of Gram stain, KOH wet mount and fungal culture in identification of fungi. Materials and Methods: After taking written and informed consent taken.

12p nguaconbaynhay3 07-02-2020 17 0   Download

Microbial contamination of the Operation theatres (OT‟s) continues to increase the prevalence of nosocomial infections. Our study evaluates the microbial contamination using various air sampling techniques in OT‟s and find a suitable technique to assess air quality. A total 151 air samples were collected from various OT‟s of our tertiary care centre.76 samples were collected by an active method using an air slit sampler and 75 samples were collected by passive settle plate method using 5% Sheep blood agar in 9cm petri-plates.

9p nguaconbaynhay3 07-02-2020 11 0   Download