Agar plates

This study aims to evaluate the microbial contamination level and identify bacterial colonization of the operating room in the hospital. Methods: Three sampling procedures were used in this study, which includes swabbing, air sampler and contact agar plate. Collected samples from 8 ORs were transported and microbiologically processed using standard procedures.

6p vischultz 17-10-2023 3 2   Download

S. sclerotiorum isolates were obtained from flower petals of commercial rapeseeds field in Hul, Slovakia (2018) by transferring a single hyphal tip to the plates with potato dextrose agar (PDA). Two sets of 20 isolates were paired in all possible combination and cultivated on PDA amended by bromophenol blue. Mycelial interactions were scored after 7 days.

4p mudbound 10-12-2021 4 1   Download

Liquid cultures have been commonly used in space, toxicology, and pharmacology studies of Caenorhabditis elegans. However, the knowledge about transcriptomic alterations caused by liquid cultivation remains limited. Moreover, the impact of different genotypes in rapid adaptive responses to environmental changes (e.g., liquid cultivation) is often overlooked. Here, we report the transcriptomic and phenotypic responses of laboratory N2 and the wild-isolate AB1 strains after culturing P0 worms on agar plates, F1 in liquid cultures, and F2 back on agar plates.

14p vitzuyu2711 29-09-2021 9 1   Download

This study was proposed to determine the recovery rate of Aspergillus species in Ankara, Turkey, from soil and their identification as well as to evaluate the proteolytic activity of the isolated strains. 20 different soil samples were collected and searched for the presence of the fungal species after selective plating and internal transcribed spacer (ITS) gene region sequencing of cPCR fragments.

9p cothumenhmong11 11-05-2021 13 1   Download

The study was carried out to investigate the effect of hatching egg fumigation and nest box management on egg hygiene and chick quality. Eggs collected from two randomly selected farms were fumigated in the farm itself. Egg shell impression was made on selective agar plates before and after fumigation to study the shell microbial load from nest box.

4p chauchaungayxua10 18-03-2021 9 1   Download

Mycoparasitism by Trichoderma sp. is one of the important modes of action exhibited by biocontrol agents against host fungi including plant pathogenic ones. Its study in-vitro constitutes considerable importance while studying bio-efficacy of an antagonist against a fungal pathogen. At present there is no standard, rapid, easy and reliable method to study mycelial interaction between a possible mycoparasitic fungi and host.

5p angicungduoc8 07-11-2020 6 1   Download

The experiments were conducted at Department of Plant Pathology, S.K.N. College of Agriculture, Jobner (Rajasthan). Five samples of blonde psyllium seeds were collected from Barmer, Nagaur, Pali, Jodhpur and Jalore districts of Rajasthan and tested for seed borne fungi. Both Blotter and Agar Plate methods were used to detect the maximum number of mcontrol and external seed borne fungi. A total of 5 species of fungi were identified using the two selective methods. The pathogencity of A. alternata was studied on psyllium seeds and seedlings.

6p nguaconbaynhay8 13-10-2020 14 2   Download

The objective of present study was to screen and isolate protease producing bacteria from soil samples collected from rhizospheric soil of apple orchard from Kotkhai of Shimla District. Collected soil samples were serially diluted and 0.1ml of sample was spread on skim milk agar plates at 370C for 48 hrs. Out of fifteen, six bacterial colonies from rhizospheric soil samples showed clear zone around the colony indicating protease activity.

7p trinhthamhodang7 31-08-2020 14 2   Download

In light of the same, the objectives of this study include to isolate various Staphylococcus species from clinical specimens. And also to compare three phenotypic methods for detection of biofilm formation among the isolated Staphylococcus species, namely, Tube adherence method, Tissue culture plate method and Congo red agar method.

9p nguaconbaynhay7 15-08-2020 13 1   Download

The aim of this study was to detect biofilm formation by study isolates of Pseudomonas aeruginosa qualitatively and quantitatively. Ten isolates were taken from diseased animal in veterinary clinics of RAJUVAS Bikaner. Qualitative biofilm formation assays (Pellicle formation assay and Congo red agar method) and quantitative assay by Microtitre plate assay used for biofilm production.

6p nguaconbaynhay7 15-08-2020 13 1   Download

Kunun-zaki are locally produced beverages which of recent have become popular among the various non-alcoholic drinks in the northern part of Nigeria; Viable bacterial counts were taken before and after the addition of soymilk on MRS agar plates after incubating anaerobically at 37 °C for 48 hours. Distinct colonies were purified for identification. kunun-zaki was fortified with soymilk, and subjected to sensory tests after 48h of incubation.

10p nguaconbaynhay7 15-08-2020 7 1   Download

The milk samples (Raw and Boiled) were collected from various vendors of Hoshiarpur and Ludhiana district. Three different samples of Pasteurized milk samples were taken from the market. These samples were evaluated for their quality by determining Standard Plate Count (SPC) and Coliform count. The bacterial colonies ranged from 0.7×104 cfu/ml to 6×106 cfu/ml in boiled milk. Methylene Blue reduction test (MBRT) had also been performed to check microbial quality of the samples and Sample 1 (both raw and boiled) was reported to be poor.

6p angicungduoc6 22-07-2020 11 1   Download

The role of currency as reservoirs of pathogenic bacteria and UV light to disinfect the contaminated notes was evaluated. Two hundred currencies of different denomination were collected from different places from central part of Karnataka. The sterile swab was rolled over the currency and dipped in a falcon tube containing 10 ml of brain heart infusion broth. After 24 hours, subculture was done on Blood agar, Mac Conkey agar plates and SDA tube. After incubation for 24-48 hours, plates were examined for the growth and microorganisms were identified by standard microbiological techniques.

7p angicungduoc6 22-07-2020 10 1   Download

Brucellosis is an infectious disease, has a considerable impact on animal health as well as socioeconomic impacts. It causes significant reproductive losses in sexually mature animals. The present study reports the isolation, identification and molecular detection of Brucella abortus. A total of 192 buffalo clinical samples were processed by cultural isolation on BBL Brucella agar plate with selective antibiotic supplements and direct PCR.

9p angicungduoc6 20-07-2020 19 2   Download

A novel bacterium SA13A was isolated from tannery effluent. Its morphology, physiology and 16S rRNA gene sequence were characterized. Bacterium SA13A was found to be gram negative, rod shaped and produces yellowish pigment on nutrient agar plates. The 16S rRNA gene sequence similarity indicated that isolate SA13A is associated with genus Luteimonas (99%).

9p trinhthamhodang6 09-07-2020 9 2   Download

Breast milk is the best source of nutrition as it is composed of all bio active components required for the growth and development of infant. Even after the advances in infant formula mixes, breast milk is universally accepted to be best suited to infants. The objective of this study is to ensure quality of donated breast milk as a safe end product. The study is done to determine the prevalence of potentially pathogenic micro-organism that indicates hygiene and sanitary conditions of human milk collected at milk bank.

4p chauchaungayxua6 26-06-2020 9 0   Download

In the present study attempts have been made on the isolation and identification of fungi in order to study the mycoflora responsible to reduce the seed quality, longevity and vigor in the pulse of Hyacinth bean. Lablab purpureus L. is an annual plant that grows in poorly - drained soils belongs to the family Fabaceae. It contains high amount of protein, vitamins and minerals. Fungal cultures were isolated by using blotter and agar plate methods.22 fungal species and 14 genera were isolated.

5p nguaconbaynhay6 24-06-2020 13 0   Download

The objective of the present study was to identify protease producing bacteria isolated from food processing industries. Isolation of the organism was performed by serial dilution agar plate technique and initial screening of protease production was done using gelatine agar plates by flooded the plates with mercuric chloride solution (HgCl2-15g and 20 ml of 6.0 N HCl made up to 100 ml with distilled water). A total of 5 isolates were selected based on zone diameter in gelatine clear zone method and named as TKMFT8, TKMFT22, TKMFT25, TKMFT39 and TKMFT53.

12p nguaconbaynhay6 24-06-2020 14 1   Download

Urinary Tract Infections are among the most common bacterial infections and account for a significant part of the workload in clinical microbiology laboratories. The rate of yield is almost same for all media i.e., Blood Agar, MacConkey gar, C.L.E.D Agar with Andrade’s indicator and Hichrome UTI Agar. The results obtained after various specific biochemical tests and other techniques, were correlated correctly with the presumptive identification results by Hicrome UTI Agar.

7p nguaconbaynhay6 24-06-2020 17 0   Download

Objective of the research was to validate the potential of Syzygium aromaticum and Kaempheria galanga against biofilm formation and plasmid borne drug resistance exhibited by Pseudomonas spp. Antimicrobial property of S.aromaticum and K.galanga on P.aeruginosa culture was assessed using the agar well diffusion method. Crystal violet assay was employed to determine the percentage inhibition of P.aeruginosa biofilm by the extracts following generation of biofilm on multiwell plates.

7p nguaconbaynhay6 23-06-2020 30 0   Download