Amur carp

A reversed phase high performance liquid chromatography (HPLC) method with UV detection by multi-step gradient elution method has been developed for simultaneous determination of testosterone, 17β-estradiol, 17α, 20β-dihydroxy-4- pregnen-3-one and cortisol in fish. The method described includes limited sample preparation with single step SPE using C18 cartridge.

8p quenchua4 16-04-2020 13 0   Download

Physiological changes corresponding to sex, age group and seasons are connected with the reproduction stages of fish. Gonadosomatic indices (GSI), hepatosomatic indices (HSI) Fulton’s condition factor (K) and somatic condition factor (Ks) of two age groups (2+ and 1+ year age) of female Amur common carp were measured during different seasons (spring, summer, autumn and winter). Significant differences (p< 0.05) were observed for both the age groups in relation to age, seasons and interaction (age and seasons) for GSI, HSI, K and Ks level.

8p nguaconbaynhay3 07-02-2020 11 1   Download

The role of testosterone in reproductive performance of Amur common carp has been studied during different seasons using the RP-HPLC method from gonad and plasma of two different age groups of both sexes. Gonadal and plasma T levels in both the age groups showed similar seasonal trend with highest level in spring season, which decreased in summer and continued to decrease to the lowest level in autumn followed by slight increase in winter season. Statistically significant differences (p< 0.

6p nguaconbaynhay3 07-02-2020 21 0   Download