Antibiotic resistance mechanisms

Mutation acquisition is a major mechanism of bacterial antibiotic resistance that remains insufficiently characterised. Here we present RM-seq, a new amplicon-based deep sequencing workflow based on a molecular barcoding technique adapted from Low Error Amplicon sequencing (LEA-seq).

15p vibransone 28-03-2024 7 2   Download

Colistin is considered the last-line antibiotic against multidrugresistant Gram-negative bacterial infections. The global dissemination of Mobile colistin resistance mcr-harboring bacteria is threatening public health. Such isolates have been detected in various reservoirs including animals, food products, the environment, and humans.

12p vibego 02-02-2024 5 0   Download

Part 2 book "Encyclopedia of meat sciences (Vol 2)" includes content: Meat research institutions, meat species determination, mechanically recovered meat, microbial contamination, minced meats, microbiological analysis, microbiological safety of meat, microorganisms and resistance to antibiotics, modeling in meat science, muscle fiber types and meat quality, nutrient claims on packaging,... and other contents.

243p muasambanhan05 22-01-2024 3 2   Download

Methicillin-resistant Staphylococcus aureus (MRSA) is a threat to global health due to its resistance to β-lactam antibiotics and many other antibiotic classes developed via both intrinsic and acquired mechanisms. In this study, molecular characteristics related to antibiotic resistance of MRSA strain BM85 were investigated by whole-genome sequencing of a sample isolated from a patient with bloodstream infection at Bach Mai Hospital, Vietnam.

10p vimulcahy 18-09-2023 3 3   Download

In this study, we assessed the status of antibiotic resistance of H. pylori isolates to antimicrobial agents at Hospital of Hue University of Medicine and Pharmacy in Central Vietnam and identified the underlying molecular mechanisms related to tetracycline and amoxicillin resistance.

8p vicaptainmarvel 21-04-2023 4 2   Download

Fitness costs imposed on bacteria by antibiotic resistance mechanisms are believed to hamper their dissemination. The scale of these costs is highly variable. Some, including resistance of Staphylococcus aureus to the clinically important antibiotic mupirocin, have been reported as being cost-free, which suggests that there are few barriers preventing their global spread.

12p vigalileogalilei 27-02-2022 14 2   Download

Antimicrobial resistance (AMR) threatens millions of people around the world and has been declared a global risk by the World Economic Forum. One of the important AMR mechanisms in Enterobacteriaceae is the production of extended-spectrum β-lactamases. The most common ESBL, CTX-M β-lactamases, is spread to the world by CTX-M-15 and CTX-M-14. Sulbactam, clavulanic acid, and tazobactam are first-generation β-lactamase inhibitors and avibactam is a new non-β-lactam β-lactamase inhibitor.

12p tudichquannguyet 29-11-2021 6 1   Download

Typhoid fever is an acute systemic infection of humans caused by Salmonella enterica subspecies enterica serovar Typhi (S. Typhi). In chronic carriers, the bacteria survive the harsh environment of the gallbladder by producing biofilm. The phenotype of S. Typhi biofilm cells is significantly different from the free-swimming planktonic cells, and studies have shown that they are associated with antibiotic resistance, immune system evasion, and bacterial persistence. However, the mechanism of this transition and the events leading to biofilm formation are unknown.

9p vilarryellison 29-10-2021 11 1   Download

Glycopeptide antibiotics inhibit bacterial cell-wall synthesis, and are important for the treatment of infections caused by multi drug-resistant strains of enterococci, streptococci and staphylococci. The main mechanism by which bacteria resist the action of glycopeptides is by producing a modified cell-wall in which the dipeptide D-Alanine-D-Alanine is substituted by D-Alanine-D-Lactate or D-Alanine-D-Serine. Recently, it has been shown that inorganic phosphate (Pi) induces hypersensitivity to vancomycin in Streptomyces coelicolor (which is highly resistant to the antibiotic in low-Pi media).

16p vibeauty 23-10-2021 13 1   Download

The molecular mechanisms underlying bacterial cell death due to stresses or bactericidal antibiotics are complex and remain puzzling. Due to the current crisis of antibiotic resistance, development of effective antibiotics is urgently required. Previously, it has been shown that iron is required for effective killing of bacterial cells by numerous bactericidal antibiotics.

13p vitzuyu2711 29-09-2021 6 1   Download

Osteomyelitis is the infection and inflammation of the bone. Inappropriate use of antibiotics and multidrug resistance has raised the morbidity and mortality rate in chronic osteomyelitis. This study aims to determine the bacterial profile and antimicrobial susceptibility patterns of chronic osteomyelitis with special mention to various resistant mechanisms. Samples from osteomyelitis cases were aerobically cultured and isolates from culture positives were identified by standard procedures. Antimicrobial susceptibility testing was done by Kirby Bauer disk diffusion method.

9p chauchaungayxua12 29-07-2021 13 2   Download

The emergence of multi-drug resistant pathogens is an urgent health-related problem, and the appropriate use of antibiotics is imperative. It is often difficult to identify the causative bacteria in patients with aspiration pneumonia because tracheal aspirate contains contaminants of oral bacteria.

12p vimaine2711 26-03-2021 10 2   Download

Several studies demonstrated the feasibility of predicting bacterial antibiotic resistance phenotypes from whole-genome sequences, the prediction process usually amounting to detecting the presence of genes involved in antibiotic resistance mechanisms, or of specific mutations, previously identified from a training panel of strains, within these genes.

11p viconnecticut2711 28-10-2020 11 1   Download

Carbapenems are one of the β-Lactam antibiotics with a broad spectrum of antibacterial activity. Inappropriate use of this antibiotic can produce resistance strains by production of carbapenemases enzyme. The Klebsiella pneumoniae carbapenemase (KPC) is the most important mechanism of enzymatic resistance seen in Enterobacteriaceae isolated especially Klebsiella pneumoniae. The aim and objectives of this study was isolation and detection of the Carbapenem resistant Klebsiella species strains with phenotypic methods. Out of 11248 samples, 602 Klebsiella species (33.

6p nguaconbaynhay8 13-10-2020 11 1   Download

The aim of this study was to support for finding novel mechanisms that render bacteria resistant to the ribosome targeting antibiotics, we screen for antibiotic resistant 16S and 23S ribosomal RNAs (rRNAs) in multidrug resistant Salmonella serovars isolated from raw retail meats isolated from Hanoi, Vietnam. Bioinformatic analysis identified 193 unknown novel mutations (64 mutations in 16S rRNA and 129 mutations in 23S rRNA genes).

8p nguyenxuankha_bevandan 14-08-2020 20 3   Download

Bacterial multidrug resistanceis a phenomenon that has been growing with increasing speed worldwide. New mechanisms of resistance have been described in different bacterial pathogens, representing a threat to effective treatments of common infectious diseases with currently available drugs. The use of drugs known as "non-antibiotics are among the alternative approaches to the use of conventional antibiotics. This approach has been studied in order to counteract the global threat of multidrug resistance. This group of drugs includes various non-steroidal anti-inflammatory drugs (NSAIDs).

12p quenchua6 15-06-2020 11 3   Download

Use of antibiotics as a therapeutic measure is the most used method for the treatment of infectious diseases including in fisheries and aquaculture. The successful use of any antibiotic in aquaculture is compromised due to the emergence of antimicrobial resistance in bacteria. Bacteria may be intrinsically resistant to different antimicrobial agents or they may acquire resistance through horizontal gene transfer. A number of biochemical and physiological mechanisms are responsible for the development of this resistance.

16p cothumenhmong5 17-05-2020 9 2   Download

Trichoderma fungi live in the soil rhizosphere and are beneficial for plant growth and pathogen resistance. Several species and strains are currently used worldwide in co-cultivation with crops as a biocontrol alternative to chemical pesticides even though little is known about the exact mechanisms of the beneficial interaction.

11p vichoji2711 29-04-2020 16 1   Download

Sounds strange that bacteria can talk to each other, though not verbally like humans but with the help of chemical signals which bacteria produce by forming biofilm, producing virulence factors or developing antibiotic resistance. The process starts only when the bacterial cell reach a threshold density enough to produce signals which can be detected by the receptors.

8p angicungduoc4 26-04-2020 14 1   Download

Antimicrobial resistance is one of the major threats to human health. Thereby, discovering alternative strategies that can circumvent the mechanisms of resistance presents a big challenge. One of which is the use of nanotechnology and Photodynamic therapy.

14p nguathienthan4 21-04-2020 11 1   Download