Aseptic culture

Anther culture is an effective breeding method for improving desirable traits, since it allows fast achievement of genetically homozygous lines. The present studies were carried out to evaluate the response of flue-cured tobacco anthers for high frequency pollen embryo induction in different culture media, and the effects of colchicine treatment on tobacco chromosome doubling. The results showed that different media caused significant changes in pollen embryo induction, in which the most obvious change was found in B medium with induction rates of 23.28%, 26.64%, and 28.

9p tudichquannguyet 29-11-2021 5 1   Download

Klebsiella is an opportunistic bacterial pathogen of man and animals known for presence of diversity of Anti Microbial Resistance (AMR). The present study was undertaken to isolate and study the anti-microbial resistance in Klebsiella isolates from cattle and buffaloes. Sixty (60) samples, comprising of 33 mastitic milk and 27 diarrhoeic faecal samples were collected aseptically from cattle and buffaloes. From these 60 samples, 11 (18.33%) Klebsiella species were isolated and identified on the basis of cultural, morphological and biochemical characteristics.

5p caygaocaolon11 21-04-2021 10 2   Download

In the present study, faecal and blood samples were collected, aseptically, from an elephant arrived to the Veterinary Clinics of the College. Immediately, samples were used for bacterial isolation. A bacterial isolate, isolated from the faecal sample of an elephant was identified at the genus level to be of Escherichia species by adopting Enterobacteriaceae identification kit used for studying biochemical characteristics and carbohydrate utilization tests (Gram negative bacilli showing mainly ONPG positive, oxidase negative and nitrate reduction positive).

7p chauchaungayxua11 23-03-2021 15 2   Download

The present experiment was conducted to standardize the protocol for in vitro mutagenesis of gladiolus hybridus varieties ‘Punjab Glance’ and ‘Sylvia’. The nodal buds were irradiated with different doses of gamma rays (Co60) 0, 5, 8, 10 and 12 Gy. Irradiated explants were then cultured aseptically on MS basal medium supplemented with 5 mg/l (BAP) + 2mg/l (IBA) and agar-agar to induce shoot and root proliferation.

11p gaocaolon9 22-12-2020 9 3   Download

The aim of this study was to investigate the prevalence of positive microbiology samples after osteosynthesis of proximal humerus fractures at the time of revision surgery and evaluate clinical characteristics of patients with positive culture results.

6p vimariana2711 22-12-2020 14 1   Download

In view of importance of Quince in the state economy and need for its rapid multiplication, the present investigation was laid to develop a commercial micropropagation protocol of Cydonia oblonga mill cv.SKAU-016.

9p caygaocaolon8 07-11-2020 14 2   Download

Pre-requisite for any plant biotechnological approach is development of an efficient plant regeneration system in that crop. In this regard the in vitro regeneration was achieved by using cotyledon explants from aseptically raised seedlings of popular Capsicum F1 hybrids Bharat and Indra. Seeds of both hybrids were exposed to different treatments for proper germination, were decontaminated and sown in vitro on half-strength MS medium.

13p kequaidan7 01-09-2020 11 2   Download

The study was conducted to standardize an efficient protocol for callus induction, subsequent growth and in-vitro regeneration in six genotypes of proso millet viz., Gavhe, Sakhroli, Kalsuli, Vari No.10, Asond and Sukdhar. The seeds were surface sterilized with 70% ethanol for 1 minute + 1% HgCl2 for 10 minutes recorded maximum aseptic culture establishment in all genotypes. The mature embryo (seeds) of proso millet was cultured on MS medium supplemented with varying concentrations of 2,4-D (0.1-2.0 mg/l) and NAA (1.0-4.

11p angicungduoc6 20-07-2020 13 1   Download

Plant tissue culture is the method of culturing the plant cells or tissues under controlled aseptic conditions on a solid or liquid medium. It has value in basic research for the production of true to type, disease free and micro-propagated plants. Besides this also has commercial application. This review work outlines the work done on pertaining to various aspects of in vitro micro-propagation, regeneration, rooting, hardening, in potato.

7p kethamoi6 29-06-2020 24 1   Download

Chronic suppurative otitis media (CSOM) is chronic inflammation of middle ear and mastoid cavity with recurrent ear discharge lasting for more than 6months. Studies are mainly done on the bacterial aetiology of CSOM, but very little is known about the fungal aspects of such cases. Otomycosis is increasing in the recent years because of the indiscriminate use of broad-spectrum antibiotics, corticosteroids, and an increase in the number of immune deficiency disorders.

6p nguaconbaynhay6 24-06-2020 14 0   Download

India accounts for 30% of neonatal deaths globally. Bacterial sepsis is a significant cause of morbidity and mortality in newborns. The study helps to make antibiotic policy in neonatal sepsis. The main objective is to study the incidence of multidrug resistant gram negative and gram positive organisms causing neonatal septicemia and their antibiotic sensitivity pattern. The study was conducted in the Department of Microbiology over a period of one year. Sample of blood was collected under aseptic precautions and processed by standard techniques.

5p gaocaolon5 14-06-2020 20 2   Download

Brahmi (Bacopa monnieri L.) belonging to the family Scrophulariaceae is an endangered perennial, glaburous, succulent creeping herb of great medicinal importance. Increased demand of Bacopa based drugs makes natural populations of this herb vulnerable to overexploitation. It is therefore essential to optimize and scale up in vitro multiplication protocols for conservation and multiplication of wild and cultivated germplasm. Nodal segments with axillary buds of an elite Bacopa genotype, were surface sterilized and inoculated to initiate aseptic cultures on 0.

12p angicungduoc5 14-06-2020 12 1   Download

Conventional propagation of Anthurium (Anthurium andraeanum [Lind.]) plant through seeds and suckers is a tedious and very slow process and not practical when carried out commercially on large scale. Hence, biotechnological approach such as micropropagation is considered as a novel method for production of pathogen-free true-to-type plantlets at a faster rate.

7p kethamoi5 03-06-2020 15 0   Download

Anthurium is an important ornamental plant grown around the globe and also in India. Seed and vegetative propagation of this plant are tedious and very slow process and not practical when carried out on a large scale. Therefore, biotechnological approach such as in- vitro cloning is found to be a novel method for production of pathogen free true-to-type plantlets at a faster rate. In this study, a competent surface sterilization procedure was standardized for aseptic in-vitro culture from leaf explants of Anthurium adraeanum (Hort.) cv. Fire under in vitro conditions.

7p trinhthamhodang5 16-05-2020 13 1   Download

Sepsis is a major cause of morbidity and mortality among neonates. Neonatal sepsis may be categorized as early-onset or late-onset. Clinical manifestations of neonatal sepsis are non-specific, therefore, clinical diagnosis of sepsis is difficult and laboratory help is required. Present study was done to evaluate three different methods in diagnosis of neonatal sepsis. 3 ml of venous blood was collected employing strict aseptic precautions from all babies admitted to the NICU in our hospital during the study period. 2 ml of blood was aseptically added to BacT/ ALERT PF culture bottle.

9p kequaidan4 21-04-2020 37 1   Download

Tungrymbai is a traditional fermented dish of the Khasi and Jaintia tribes of Meghalaya. In this study an attempt was made to standardize the preparation of “Tungrymbai” using Lactobacillus fermentum and Lactobacillus plantarum culture obtained from fermented foods of Meghalaya. Three samples were prepared and the bacterial strain was added aseptically to the cooked soybeans in a combination of 1:1 ratio at 1, 2 and 3% culture combination respectively, a traditional sample was used as control. The samples were fermented at 37˚C for 3-4 days.

12p trinhthamhodang1212 06-04-2020 18 1   Download

Corynebacterium sp. is a Gram-positive, non-motile and facultatively anaerobic bacilli present in the environment as ubiquitous organism. A 45 day-old Holstein Friesian calf from University Livestock Farm (ULF), Kerala Veterinary and Animal Sciences University, Mannuthy was reported with joint ill in both of its forelimbs. Using aseptic procedures, synovial fluid was collected from the right knee joint and inoculated on to blood agar and incubated at 37⁰ C for 24 to 48 hrs to isolate and identify the bacterial aetiology involved.

6p caygaocaolon2 11-03-2020 10 1   Download

The dermatophytes are a group of closely related fungi that have the capacity to invade keratinized tissue (skin, hair, and nails) of humans and other animals to produce an infection. The clinical importance of identifying species of dermatophytes is to find out problem source of infection. Moreover, there are some prognostic considerations as well. The study was conducted at the Upgraded department of Microbiology, Osmania general hospital, Hyderabad.

7p cothumenhmong3 22-02-2020 13 0   Download

Chromogenic medium is culture media for simple and fast detection of bacteria using chromogenic substrates, resulting in the different coloration of certain bacteria colonies. Have several advantages over traditional media, such as cysteine-lactose-electrolyte deficient (CLED) medium, allow for easier recognition of mixed growth, save time, reduce workload and provides higher detection rates. To compare between chromogenic colour agar and biochemical tests in identification of organisms causing bacteriuria that associated with urinary Schistosomiasis.

6p cothumenhmong3 22-02-2020 22 0   Download

Otomycosis is an acute, subacute or chronic fungal infection of the external auditory canal. It is worldwide in distribution with a higher prevalence in the hot, humid, and dusty areas of the tropics and subtropics. A wide variety of fungi can cause Otomycosis. In this study, our aim was to determine the most common mode of presentation, predisposing factors, and the spectrum of fungi involved in Otomycosis. 60 Ear discharge samples were collected aseptically using two sterile cotton swabs. One swab was used for direct microscopic examination and used for fungal culture.

5p trinhthamhodang3 12-02-2020 9 0   Download