Atoms and ions

Amyloid protein (Ab1–40) aggregation and conformation was examined using native and sodium dodecyl sulfate/ polyacrylamide gel electrophoresis,and the results com-pared with those obtained by atomic force microscopy, and with Congo red binding,sedimentation and turbidity assays. The amount of Ab aggregation measured was different,depending upon the method used. Incubation for 15 min at pH 5.0 or in the presence of Fe 2+ ,Cu 2+ or Zn 2+ did not alter the level of Aboligomers observed on SDS and native gels....

12p tumor12 20-04-2013 41 4   Download

Vacuolar H + -translocating inorganic pyrophosphatase [vacuolar H + -pyro-phosphatase (V-PPase); EC 3.6.1.1] is a homodimeric proton translocase; it plays a pivotal role in electrogenic translocation of protons from the cyto-sol to the vacuolar lumen, at the expense of PPi hydrolysis, for the storage of ions, sugars, and other metabolites.

14p vinaphone15 25-02-2013 26 4   Download

Onconase (ONC) is an amphibian member of the pancreatic ribonuclease superfamily that is selectively toxic to tumor cells. It is a much less efficient enzyme than the archetypal ribonuclease A and, in an attempt to gain fur-ther insight, we report the first atomic resolution crystal structure of ONC, determined in complex with sulfate ions at 100 K.

14p cosis54 09-12-2012 54 3   Download