base isolation

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most predominant agents that cause nosocomial infections. Objectives: To determine the rate of MRSA and the molecular characteristics of the coagulase encoding gene of these isolates based on polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP).

6p viuchiha 03-01-2025 1 1   Download

The authors develop a system of differential equations describing the motion of the structural system equipped with DFP bearings. The Runge-Kutta numerical method, specifically the ode15s function in MATLAB, was used to solve these equations. The results of the calculations provide a comparison of the damping efficiency of the structural system with and without the use of DFP bearings, assessing the effectiveness of vibration isolation during an earthquake, with three components X, Y, and vertical considered.

5p vibenya 31-12-2024 2 0   Download

Interface converter, for converting RS-232 to RS-422 and RS-485, with electrical isolation, 2 channels Bidirectional, wireless, 2400 MHz transmission system (European version), consisting of 2 transceivers (transmitter and receiver) and two antennas with connecting cable Fieldline Modular Wireless IO set, consisting of: Wireless IO base station for one Wireless IO terminal and Wireless IO device, 8 digital inputs, 8 digital outputs

23p buisontdtq 04-10-2012 53 9   Download

In the course of current research the procedure of obtaining of the piecewise linear vibration systems time and frequency responses have been developed. The method is partly analytical and partly numerical and is based on combining of linear solutions of different sections in a cycle of motion. In a row with the method of obtaining of the exact steady state time and frequency response, also it is provided sensitivity analysis of the vibration isolator.

86p runthenight07 01-03-2023 11 3   Download

The objectives of the thesis: Identify the pathogenicity potential and classificate at species level of Colletotrichum spp. isolated from hot chilli in Vietnam; produce stable oligochitosan-silica nano, which potentially highly induced effectively the systemic resistance against anthranose disease on hot chilli, based on the combination of low molecular weight chitosan with nano silica.

28p capheviahe27 23-02-2021 32 4   Download

The research objectives of the dissertation isolate and determine the structure of clean compounds from mondo grass root roots. Evaluate cytotoxic effects and anti-inflammatory activity based on the effect of inhibiting NO production of isolated compounds.

27p xacxuoc4321 11-07-2019 41 3   Download

Many groups of proteins play important roles in the cell’s response to various stresses. The molecular chaperone GroEL of Escherichia coli represents one such highly conserved family of stress proteins. We have observed that isolated GroEL complexes from stationary cultures contain various polypeptides that can be released from the chaperonin by GroES and/or ATP, and identified two such polypeptides as the proteins GatY and UP12.

9p system191 01-06-2013 31 3   Download

A series of trehalose-based oligosaccharides were isolated from the cytoplasmic fraction of Mycobacterium smegmatis and purified by gel-filtration and paper chromatography and TLC. Their structures were determined by HPLC and GLC to determine sugar composition and ratios, MALDI-TOF MS to measure molecular mass, methylation analysis to determine linkages, 1H-NMR to obtain anomeric configurations of glycosidic linkages, and exoglycosidase digestions followed by TLC to determine sequences and anomeric configurations of the monosaccharides....

8p system191 01-06-2013 36 5   Download

For the biosynthesis of punicic acid (18:3 D9Z,11E,13Z )a (11,14)-linoleoyl desaturase activity has been proposed. To isolate this acyl-lipid-desaturase, PCR-based cloning was used. This approach resulted in the isolationof twocomplete cDNAs. The first isolated full-length cDNA harbors a sequence of 1350 bp encoding a protein of 395 amino acids. The second cDNA was 1415 bp long encoding a protein of 387 amino acids.

8p research12 29-04-2013 41 3   Download

The photosynthetic protein complexes in plants are located in the chloroplast thylakoid membranes. These membranes have an ultrastructure that consists of tightly stackedÔgranaÕ regions interconnectedbyunstackedmembrane regions.The structure of isolated granamembranes has been studiedhere by cryo-electron microscopy. The data reveals an unusual arrangement of the photosynthetic protein complexes, staggered over two tightly stacked planes.

11p research12 29-04-2013 42 2   Download

Pi4 is a38-residue toxincross-linkedby fourdisulfidebridges that has been isolated from the venom of the Chactidae scorpion Pandinus imperator. Together with maurotoxin, Pi1, Pi7 andHsTx1, Pi4belongs to theaKTX6 subfamilyof short four-disulfide-bridged scorpion toxins acting on K + channels. Due to its very low abundance in venom, Pi4 was chemically synthesized in order to better characterize its pharmacology and structural properties.

10p tumor12 20-04-2013 31 1   Download

We have determined and compared nucleotide excision repair capability of several rat tissues by amethod based on restoration of the transformation activity of UV-irradiated pBlueScript by incubation in repair-competent protein extracts.After 3 hof incubation, plasmidDNAwas isolated and used to transform competent Escherichia colicells. Damaged plasmids showed low transformation efficiency prior to incubation in repair-competent extracts. After incubation the transformation efficiency was restored to different extents permitting calculationof the repair capacity of the extracts....

6p tumor12 20-04-2013 22 3   Download

NMR and visible spectroscopy coupled to redox measurements were used to determine the equilibrium thermodynamic properties of the four haems in cytochrome c3 under conditions in which the protein was bound to lig-ands, the small anion phosphate and the protein rubredoxin with the iron in the active site replaced by zinc.

10p awards 06-04-2013 38 4   Download

In several organisms d-cysteine desulfhydrase (d-CDes) activity (EC 4.1.99.4) was measured; this enzyme decomposes d-cysteine into pyruvate, H2S, and NH3. A gene encoding a putative d-CDes protein was identified in Arabidopsis thaliana(L) Heynh. based on high homology to an Escherichia coliprotein called YedO that hasd-CDes activity. The deduced Arabidopsisprotein consists of 401 amino acids and has a molecular mass of 43.9 kDa.

14p awards 06-04-2013 36 3   Download

The NMR solution structures of NTX-1 (PDB code 1W6B and BMRB 6288), a long neurotoxin isolated from the venom ofNaja naja oxiana, and the molecular dynamics simulation of these structures are reported. Calculations are based on 1114NOEs, 19 hydrogen bonds, 19 dihedral angle restraints and secondary chemical shifts derived from 1 Hto 13 C HSQC spectrum. Similar to other long neurotoxins, the three-finger like structure shows a double and a triple strandedb-sheet aswell as some flexible regions, particularly at the tip of loop II and the C-terminal tail....

0p awards 05-04-2013 46 2   Download

The novel sex-specific potassium channel inhibitor IsTX, a 41-residue peptide, was isolated from the venom of male Opisthacanthus madagascariensis.Two-dimensionalNMR techniques revealed that the structure of IsTX contains a cysteine-stabilizeda/b-fold. IsTX is classified, based on its sequential and structural similarity, in the scorpion short toxin familya-KTx6. Thea-KTx6 family contains a single a-helix and twob-strands connected by four disulfide brid-ges and binds to voltage-gated K + channels and apamin-sensitive Ca 2+ -activated K + channels. ...

0p awards 05-04-2013 36 3   Download

Little is known about the functions of DNA polymerasek (Pol k) recently identified in mammals. From the genomic sequence information of rice andArabidopsis, we found that Polkmay be the only member of the X-family in higher plants. We have succeeded in isolating the cDNA and recombinant protein of Polkin a higher plant, rice (Oryza sativaL. cv. Nipponbare) (OsPolk). OsPolkhad activities of DNA polymerase, terminal deoxyribonucleotidyl trans-ferase and deoxyribose phosphate lyase, a marker enzyme for base excision repair. ...

9p dell39 03-04-2013 53 3   Download

The cytochromebc1 complex of the yeastSaccharomyces cerevisiae is composed of 10 different subunits that are assembledas a symmetrical dimer in the innermitochondrial membrane. Three of the subunits contain redox centers and participate in catalysis, whereas little is known about the function of the seven supernumerary subunits.

10p dell39 03-04-2013 56 4   Download

We have isolated a nonfucosylated and three variously fucosylated neutral oligosaccharides from human milk that are based on theiso-lacto-N-octaose core. Their structures were characterized by the combined use of electrospray mass spectrometry (ES-MS) and NMR spectroscopy. The branching pattern and blood group-related Lewis deter-minants, together with partial sequences and linkages of these oligosaccharides, were initially elucidated by high-sensitivity ES-MS/MS analysis, and then their full structure assignment was completed by methylation analysis and 1 H-NMR.

15p dell39 03-04-2013 60 3   Download

We have isolated a laccase (lac1) from culture fluid ofVol-variella volvacea, grown in a defined medium containing 150lMCuSO4 , by ion-exchange and gel filtration chroma-tography. Lac1 has a molecular mass of 58 kDa as deter-mined by SDS/PAGE and an isoelectric point of 3.7. Degenerate primers based on the N-terminal sequence of purified lac1 and a conserved copper-binding domain were used to generate cDNA fragments encoding a portion of the lac1 protein and RACE was used to obtain full-length cDNA clones. ...

11p dell39 03-04-2013 56 6   Download