Bisulfite treatment

DNA methylation is an important mechanism of epigenetic gene expression control that can be passed between generations. Here, we use sodium bisulfite treatment and targeted gene enrichment to study genome-wide methylation across the three sub-genomes of allohexaploid wheat.

15p viaristotle 29-01-2022 12 0   Download

The most widely utilized approaches for quantifying DNA methylation involve the treatment of genomic DNA with sodium bisulfite; however, this method cannot distinguish between 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). Previous studies have shown that 5hmC is enriched in the brain, although little is known about its genomic distribution and how it differs between anatomical regions and individuals.

15p viaristotle 29-01-2022 6 0   Download

The combination of sodium bisulfite treatment with highly-parallel sequencing is a common method for quantifying DNA methylation across the genome. The power to detect between-group differences in DNA methylation using bisulfite-sequencing approaches is influenced by both experimental (e.g. read depth, missing data and sample size) and biological (e.g. mean level of DNA methylation and difference between groups) parameters.

16p vilichoo2711 23-06-2021 10 1   Download

Bisulfite treatment of DNA followed by sequencing (BS-seq) has become a standard technique in epigenetic studies, providing researchers with tools for generating single-base resolution maps of whole methylomes.

11p vioklahoma2711 19-11-2020 11 2   Download

Epigenetic heterogeneity within a tumour can play an important role in tumour evolution and the emergence of resistance to treatment. It is increasingly recognised that the study of DNA methylation (DNAm) patterns along the genome – so-called ‘epialleles’ – offers greater insight into epigenetic dynamics than conventional analyses which examine DNAm marks individually.

10p viflorida2711 30-10-2020 12 2   Download

Methylation is a common modification of DNA. It has been a very important and hot topic to study the correlation between methylation and diseases in medical science. Because of the special process with bisulfite treatment, traditional mapping tools do not work well with such methylation experimental reads.

8p viconnecticut2711 29-10-2020 12 1   Download

DNA methylation is an epigenetic modification that is studied at a single-base resolution with bisulfite treatment followed by high-throughput sequencing. After alignment of the sequence reads to a reference genome, methylation counts are analyzed to determine genomic regions that are differentially methylated between two or more biological conditions.

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Bisulfite sequencing is one of the major high-resolution DNA methylation measurement method. Due to the selective nucleotide conversion on unmethylated cytosines after treatment with sodium bisulfite, processing bisulfite-treated sequencing reads requires additional steps which need high computational demands.

9p vicoachella2711 27-10-2020 10 1   Download

DNA methylation is an important epigenetic mechanism of transcriptional control that plays an essential role in several cellular functions. Aberrant DNA methylation in cancer has been frequently associated with downregulation of microRNAs and protein coding genes, such as miR-200c/miR-141 cluster and E-cadherin.

10p viamsterdam 18-09-2020 12 0   Download

This books highlights the methods and mechanisms by which epigenetics with a focus on DNA methylation can be studied and its impacts on health. In the first part, the first chapter focuses on the modeling and feedback dynamics of DNA methylation, discussing mechanisms and controlling factors as well as DNA sequences pattern analyses and histone modifications and their association with disease initiation. Most methods for detecting methylated-CpG islands rely on chemical conversion of DNA by treatment with bisulfite....

258p yeutinh98 22-09-2012 57 5   Download

The second chapter discusses how DNA bisulfite treatment together with high-throughput sequencing allows determining the DNA methylation on a whole genome scale at single cytosine resolution and introduces software for analysis of bisulfite sequencing data. The third chapter presents analysis of GC3-rich genes that have more methylation targets.

142p yeutinh98 22-09-2012 53 6   Download