C perfringens type A

Clostridium perfringens is a Gram-positive anaerobic pathogen that causes multiple diseases in humans and animals. C. perfringens lack flagella but have type IV pili (TFP) and can glide on agar surfaces. When C. perfringens bacteria are placed on surfaces, they become elongated, flexible and have TFP on their surface, traits not seen in liquid-grown cells.

24p vijeeni2711 24-07-2021 5 0   Download

The current study reports the occurrence and molecular toxinotyping of Clostridium perfringens (C. perfringens) isolated from sheep and goat in Kashmir valley. A total of 462 faecal samples were collected from different organized and unorganized sheep and goat farms of the Kashmir valley.

11p cothumenhmong9 04-01-2021 11 1   Download

The molecular characterization of Clostridium perfringens in sheep for various toxinotypes was investigated in this study. A total of 147 samples collected from healthy, diarrhoeic animals and morbid material of animals suspected to have died of enterotoxaemia were screened for Clostridium perfringens (C. perfringens) toxinotypes.

7p trinhthamhodang1213 27-05-2020 14 1   Download

The objective of the study was to see the prevalence of C. perfringens as well as to detect the enterotoxigenic genes in C. perfringens strains isolated from different meat and water samples in Kashmir, India. For this 275 different meat and meat product, and water samples were analyzed by standard bacteriological technique. All the Fifty nine (59) isolates recovered from these samples were typed according to their gene by multiplex PCR assay. Out of these, 44 (74.57%) isolates were confirmed as C. perfringens type A based on the presence of alpha toxin gene (cpa).

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All Gram-negative bacteria and a few gram-positive bacteria shed membrane vesicles in suitable medium and environmental condition. The present study was undertaken to determine the influence of stages of growth and nutrient media on production of membrane vesicles (MVs) of Clostridium perfringens type A (C. perfringens type A), and molecular characterization of the same.

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Clostridium perfringens is currently classified into five types (A, B, C, D, E) based on the different toxins produced. Type A and C are known as the causative agent of enteritis and enterotoxemia in newborn and young piglets with severe intestinal lesions including edema, hemorrhage and necrosis. A multiplex PCR (mPCR) was developed in order to quickly and early determine the presence of genotypes of C.

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Clinical Manifestations Intestinal Disorders Food Poisoning C. perfringens, primarily type A, is the second or third most common cause of food poisoning in the United States (Chap. 122). The responsible toxin is thought to be a cytotoxin produced by 75% of strains isolated from cases of foodborne disease. The cytotoxin binds to a receptor on the small-bowel brush border and induces a calcium ion–dependent alteration in permeability. The associated loss of ions alters intracellular metabolism, resulting in cell death.

7p colgate_colgate 21-12-2010 67 4   Download