Cancer cell lines

The objectives of the thesis: Determine the chemical composition of three soft coral species S. nanolobata, S. conferta, S. leptoclados collected in the central sea of Vietnam; đetecting active substances with cytotoxic activity in soft corals studied, orienting their application for biomedical and pharmacological studies.

26p closefriend09 16-11-2021 15 4   Download

Demonstrating the main chemical constituents of Anodendron paniculatum (Roxb.) A.DC. Structural determination of isolated compounds; evaluation of the cytotoxic activities of isolated compounds against human cancer cell lines.

25p nguathienthan12 23-05-2021 13 3   Download

The research objectives of the thesis: Isolate and determine the chemical structure of compounds from C. candicans and C. macrophylla species collected in Vietnam. Assessment of toxic activity on some cancer cell lines: liver (Hep-G2), lung (Lu-1) and breast (MCF-7) of isolated clean compounds. Researching the chemical composition of C. candicans and C. macrophylla essential oils

29p extraenglish 24-05-2021 16 4   Download

Isolate and determine the chemical structure of compounds from C. candicans and C. macrophylla species collected in Vietnam; assessment of toxic activity on some cancer cell lines: liver (Hep-G2), lung (Lu-1) and breast (MCF-7) of isolated clean compounds, researching the chemical composition of C. candicans and C. macrophylla essential oils.

29p capheviahe26 25-01-2021 32 3   Download

The objectives of the thesis: Manufacturing multifunctional nanoparticles including: Fe3O4 nanoparticles (magnetical properties) coated with biocompatible polymers, attaching drugs (Cur, Dox) and targeted folate factor (optical properties)) that are well dispersed in water, able to target the cancer.Experiment and evaluate the effect of the nanoparticles on cancer cell lines such as HT29; HeLa; HepG2 ... and on experimental animals.

27p xacxuoc4321 09-07-2019 62 5   Download

Head and neck squamous cell carcinoma (HNSCC) is the most common and most aggressive type of head and neck cancer. Current approaches for the treatment of HNSCC are not sufficient to increase the patient survival or to reduce the high recurrence rate.

12p nutifooddau 27-01-2019 25 2   Download

The spermine analogue N1,N11-diethylnorspermine (DENSPM) efficiently depletes the cellular pools of putrescine, spermidine and spermine by down-regulating the activity of the polyamine biosynthetic enzymes and up-regulating the activity of the catabolic enzyme spermidine/ spermine N1-acetyltransferase (SSAT). In the breast cancer cell line L56Br-C1, treatment with 10 lM DENSPM induced SSAT activity 60 and 240-fold at 24 and 48 h after seeding, respectively, which resulted in polyamine depletion. ...

7p research12 01-06-2013 53 5   Download

Matrilysin (matrix metalloproteinase-7) plays important roles in tumor progression. It was previously found that matrilysin binds to the surface of colon cancer cells to promote their metastatic potential. In this study, we identified annexin II as a novel membrane-bound substrate of matrilysin. Treatment of human colon cancer cell lines with active matrilysin released a 35 kDa annexin II form, which lacked its N-terminal region, into the culture supernatant. The release of the 35 kDa annexin II by matrilysin was significantly enhanced in the presence of serotonin or heparin....

14p research12 29-04-2013 41 2   Download

We previously demonstrated inmast cell lines RBL2H3 and FMA3 that tryptophan hydroxylase (TPH) undergoes very fast turnover driven by 26S-proteasomes [Kojima, M., Oguro, K., Sawabe, K., Iida, Y., Ikeda, R., Yamashita, A., Nakanishi, N. & Hasegawa, H. (2000)J.Biochem (Tokyo) 2000,127, 121–127]. In the present study, we have examined an involvement of TPH phosphorylation in the rapid turn-over, using non-neural TPH.

9p research12 29-04-2013 38 3   Download

Eosinophil cationic protein (ECP), one of the major components of basic granules of eosinophils, is cytotoxic to tracheal epithelium. However, the extent of this e€ect on other cell types has not been evaluatedin vitro.Inthis study, we evaluated the e€ect of ECP on 13 mammalian cell lines. ECP inhibited the growth of several cell lines including those derived from carcinoma and leukemia in a dose-dependent manner.

10p research12 29-04-2013 36 4   Download

The effect of insulin on cancer metastatic potential was studied in a human hepatocarcinoma cell line, H7721. Cell adhesion to human umbilical vein endothelial cells (HUVECs) and lamininaswell as chemotactic cellmigration and invasion were selected as the indices of metastasis-related phenotypes for assessment of metastatic potential ex vivo. The results indicated that insulin enhanced all of these metastasis-related phenotypes.

11p tumor12 20-04-2013 41 4   Download

Asheat shockproteins (Hsps) are involved inprotecting cells and also in the pathophysiology of diseases such as inflam-mation, cancer and neurodegenerative disorders, modula-tors ofHsp expression inmammalian cells would seemto be useful for the treatment of various diseases. In this study, we isolated mammalian cell lines for screening of Hsp modu-lators; mouse C3H10T1/2 cells stably transfected with a plasmid containing the mouse Hsp105 or human Hsp70B promoter upstream of a luciferase or b-galactosidase reporter gene, respectively....

8p tumor12 20-04-2013 30 5   Download

GToligomers, showingadose-dependent cytotoxic effect on a variety of human cancer cell lines, but not on normal human lymphocytes, recognize and form complexes with nuclear proteins.By working with human T-lymphoblastic CCRF-CEM cells and by using MS and SouthWestern blotting, we identified eukaryotic elongation factor 1 alpha (eEF1A) as the main nuclear protein that specifically recognizes these oligonucleotides.Western blotting and supershift assays confirmed the nature of this protein and its involvement in forming a cytotoxicity-related complex (CRC)....

12p tumor12 20-04-2013 34 1   Download

PSA (prostate-specific antigen), the most useful serum marker for prostate cancer, is encoded by thehKLK3gene and is present in the serumas amixture of several molecular species. This work was performed to identify the hKLK3 transcripts in order to determine how many proteins resembling PSA are synthesized from thehKLK3gene and secreted in blood. Combined Northern blotting, molecular cloninganddatabase searching showed that thehKLK3gene produces at least 15 transcripts ranging in size from 0.7 to 6.1 kb. Polysomal distribution analysis revealed that the transcripts shorter than 3.

9p tumor12 20-04-2013 55 2   Download

TheST2gene, which is specifically induced by growth sti-mulation in fibroblasts, encodes interleukin-1 receptor-rela-tedproteins and iswidely expressed in hematopoietic, helper T, and various cancer cells. However, the physiological as well as pathological functions of the ST2 gene products are not yet fully understood. In this study, we analyzed the expression of theST2gene in human glioma cell lines and human brain tumor samples with real-time polymerase chain reactionmethod,

8p tumor12 20-04-2013 48 4   Download

Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) has attractedmuch attention because of its ability to kill tumour cells. In this study, we demonstrated that treatment of QGY-7703 cells with the combination of TRAIL and etoposide resulted in synergistic cytotoxic effects. In dissecting the mechanism underlying this syner-gistic effect, we found that treatment with etoposide alone resulted in the upregulation of Bax, while the level of trun-catedBid (tBid)wasunchanged.

11p fptmusic 16-04-2013 37 3   Download

Class II MHC genes (for example, the humanHLA-DRAgene) are expressed at the cell surface in many professional and nonprofessional anti-gen-presenting cells in a variety of anatomical locations. Here, we report about 13 mouse transgenic lines (11 of which have not been previously des-cribed) generated with four distinct sets of DRAtransgenes carrying pro-gressive, informative 5¢ and 3¢ deletions.

13p fptmusic 11-04-2013 34 2   Download

The aromatic hydrocarbon receptor (AhR)-dependent pathway involved in 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced immunotoxicity has been studied extensively, but the AhR-independent molecular mechan-ism has not. In previous studies we found that the AhR is not expressed in L-MAT, a human lymphoblastic T-cell line. In this report, we provide the following evidence that the protein kinase C (PKC)hactivity is func-tionally involved in the AhR-independent signal transduction mechanism that participates in the TCDD-induced L-MAT cell apoptosis....

13p awards 05-04-2013 29 2   Download

Discovery-driven translational research in breast cancer is moving steadily from the study of cell lines to the analysis of clinically relevant samples that, together with the ever increasing number of novel and powerful tech-nologies available within genomics, proteomics and functional genomics, promise to have a major impact on the way breast cancer will be diag-nosed, treated and monitored in the future.

14p awards 05-04-2013 42 3   Download

Licensing origins for replication upon completion of mitosis ensures genomic stability in cycling cells. Cdt1 was recently discovered as an essential licensing factor, which is inhibited by geminin. Over-expression of Cdt1 was shown to predis-pose cells for malignant transformation. We show here that Cdt1 is down-regulated at both the protein and RNA level when primary human fibroblasts exit the cell cycle into G0, and its expression is induced as cells re-enter the cell cycle, prior to S phase onset. ...

11p awards 05-04-2013 28 3   Download