Cell tissue culture

Understanding the metabolic changes in colorectal cancer (CRC) and exploring potential diagnostic biomarkers is crucial for elucidating its pathogenesis and reducing mortality. Cancer cells are typically derived from cancer tissues and can be easily obtained and cultured.

13p vishanshan 27-06-2024 1 1   Download

Ebook "Plant cell culture protocols (Third edition)" is taught around the world as part of undergraduate and graduate courses in agronomical and biological sciences. However, the apparent simplicity of cell culture technology should not lead to mistakingly consider it as trivial. It would be very diffi cult to understand modern plant biotechnology without in vitro cell cultures and even today, the application of recombinant DNA technology to important crops is hampered by the poor embryogenic or morphogenetic response of in vitro cultured tissues.

445p dongmelo 27-05-2024 5 2   Download

Eucalyptus is one of the important plant species for paper pulp, fuel wood, and timber. In this study, a hybrid Eucalyptus, U16 line, was investigated for rapid in vitro propagation. The apical and adventitious shoots served as initial explants. These were free-disease symptoms that were collected for surface sterilization.

8p vijaychest 24-04-2024 3 2   Download

Preclinical studies require models that recapitulate the cellular diversity of human tumors and provide insight into the drug sensitivities of specific cellular populations. The ideal platform would enable rapid screening of cell type-specific drug sensitivities directly in patient tumor tissue and reveal strategies to overcome intratumoral heterogeneity.

15p vibransone 28-03-2024 3 2   Download

Human pancreatic islets are a central focus of research in metabolic studies. Transcriptomics is frequently used to interrogate alterations in cultured human islet cells using single-cell RNA-sequencing (scRNAseq). We introduce single-nucleus RNA-sequencing (snRNA-seq) as an alternative approach for investigating transplanted human islets.

17p vibransone 28-03-2024 4 2   Download

Part 1 book "Biomedical tissue culture" includes content: Culture conditions and types of growth media for mammalian cells, cell handling and culture under controlled oxygen concentration, contamination of tissue cultures by mycoplasmas, isolation of breast cancer stem cells by single cell sorting, in vitro, tissue based models as a replacement for animal models in testing of drugs at the preclinical stages, use of cell culture to prove syncytial connection and fusion of neurons.

123p muasambanhan07 20-02-2024 3 0   Download

Part 2 book "Biomedical tissue culture" includes content: Placental structure and biological aspects of fetal membranes cultured in vitro; tissue development and mechanical property in the regenerated cartilage tissue; the art of animal cell culture for virus isolation; tissue based model of HCV replication as a replacement for animal models in drug testing; tissue culture to assess bacterial enteropathogenicity; viral DNA and CDNA array in the diagnosis of respiratory tract infections.

124p muasambanhan07 20-02-2024 2 0   Download

Ebook "Disposable bioreactors (Advances in biochemical engineering/biotechnology, Volume 115)" includes eight contributions from renowned experts, is to give an up-to-date overview of the main disposable bioreactors, their working principles, characteristics, known engineering aspects, and potential applications. R. Brecht discusses disposable bioreactor technology development in pharmaceutical glycoprotein manufacturing from the view of the applier (CMO).

228p manmanthanhla0201 26-02-2024 7 1   Download

Ebook "Porous polymeric bioresorbable scaffolds for tissue engineering" discusses the manipulation of polymer chemical structure and composition to tune scaffold physical properties, and optimization of scaffold 3D architecture by a smart use of both fabrication techniques. The multidisciplinary nature of this research is imperative in pursuing the challenge of tissue regeneration successfully.

132p manmanthanhla0201 26-02-2024 1 1   Download

Ebook "Tissue engineering III: Cell-surface interactions for tissue culture" would like to thank all authors for their excellent contributions to this volume addressing the exciting field of cell–surface interactions, with special focus on tissue generation.

349p nhanphanguyet 28-01-2024 4 2   Download

New concepts for a more efective anti-cancer therapy are urgently needed. Experimental faws rep‑ resent a major counter player of this development and lead to inaccurate and unreproducible data as well as unsuc‑ cessful translation of research approaches into clinics. In a previous study we have created epithelial cell cultures from head and neck squamous cell carcinoma (HNSCC) tissue.

13p vileonardodavinci 23-12-2023 2 2   Download

Myxofbrosarcoma is a rare malignant soft tissue sarcoma characterised by multiple local recurrence and can become of higher grade with each recurrence. Consequently, myxofibrosarcoma represents a burden for patients, a challenge for clinicians, and an interesting disease to study tumour progression. Currently, few myxofibrosarcoma preclinical models are available.

13p vileonardodavinci 23-12-2023 4 2   Download

Despite their heterogeneity, the current standard preoperative radiotherapy regimen for localized highgrade soft tissue sarcoma (STS) follows a one fits all approach for all STS subtypes. Sarcoma patient-derived threedimensional cell culture models represent an innovative tool to overcome challenges in clinical research enabling reproducible subtype-specific research on STS.

10p visharma 20-10-2023 4 2   Download

Part 1 book "Animal cell culture - Essential methods" includes content: The cell culture laboratory, sterilization, microscopy of living cells, basic techniques and media, the maintenance of cell lines, and safety, development and optimization of serum and protein free culture media.

214p oursky04 14-09-2023 5 3   Download

Part 2 book "Animal cell culture - Essential methods" includes content: Cryopreservation and banking of cell lines, primary culture of specific cell types and the establishment of cell lines, cloning, the quality control of animal cell lines and the prevention, detection and cure of contamination, systems for cell culture scale up, good laboratory practice in the cell culture laboratory.

166p oursky04 14-09-2023 5 3   Download

Graphene oxide (GO) has been attracting tremendous attention in the past few years in various biomedical applications including; tissue engineering, cell culture, biosensing, bioimaging and therapeutics delivery due to unique features it possesses. In this thesis, we demonstrate the preparation of crumpled GO functionalized with polyethyleneimine (PEI) (CGO-PEI) via surface acoustic waves (SAW) to develop a nanotherapeutic delivery platform with efficient cellular uptake and good biocompatibility.

60p runthenight04 02-02-2023 3 1   Download

Particular somatic cells, namely cumulus cells (CCs) that support the oocyte maturation, fertility, and viability by providing the nutrients and energy to the oocyte envelop the mammalian oocyte. In this study, discarded human cumulus tissues were used to reveal the value of hyaluronic acid-rich CCs on several cellular events, including differentiation. Conditioned media, recovered from the primary culture of CCs, were introduced to the human nucleus pulposus cells (hNPCs) which were functionally distorted because of the loss of chondrogenecity

16p lyhuyenthu 31-01-2023 9 2   Download

The purpose of this study was to synthesize S-protected thiolated hyaluronic acid (HA) and to evaluate its potential for 3D cell culture scaffold. S-protected thiolated HA was synthesized by the covalent attachment of Nacetyl-S-((3-((2,5-dioxopyrrolidin-1-yl)oxy)-3-oxopropyl)thio)cysteine hydrazide ligand to the HA.

9p viginny 30-12-2022 9 2   Download

Umbilical cord-derived mesenchymal stem cells have been determined to be effective in cell therapy and regenerative medicine. Cell cryopreservation is widely used for long term preservation. However, cell cryopreservation is costly and labor consume.

6p vimelindagates 18-07-2022 15 3   Download

In this article, chitosan/biphasic calcium phosphate (CS/BCP) nanofibers were prepared by electrospinning. From the culture of osteogenic cells, the biocompatibility of CS/BCP nanofibrous substrates was identified and increased by the photocrosslinking.

7p visherylsandber 04-07-2022 9 2   Download