Chilli through RAPD marker

In consequence, discriminating between elite lines and their hybrids is often difficult. The present work reports the results of applying both methods, RAPDs and ISSR, to assess the genetic purity of hybrid seeds.

8p cothumenhmong9 18-01-2021 8 2   Download

Molecular variation in chilli fruit rot pathogen (Alternata alternata) was analyzed by using six isolates collected from major chilli growing region of Maharashtra. The genomic DNA extracted from each isolate of A. alternata was subjected to polymerase chain reaction using 15 primers of OPA and OPB series off which 10 primers produced 83 scorable bands with size ranging from Among the RAPD primer 83 bands 80 bands were polymorphic and level of polymorphism was 96.38%. Molecular diversity using RAPD marker showed that the Aa2 (Satara) having higher similarity index with Aa4 (Akola).

7p trinhthamhodang1212 06-04-2020 10 1   Download