Condensation domain

Chromatin interactions are important for gene regulation and cellular specialization. Emerging evidence suggests many-body spatial interactions play important roles in condensing super-enhancer regions into a cohesive transcriptional apparatus. Chromosome conformation studies using Hi-C are limited to pairwise, population-averaged interactions; therefore unsuitable for direct assessment of many-body interactions.

17p viarchimedes 26-01-2022 13 0   Download

Phomafungin is a recently reported broad spectrum antifungal compound but its biosynthetic pathway is unknown. We combed publicly available Phoma genomes but failed to find any putative biosynthetic gene cluster that could account for its biosynthesis.

18p visilicon2711 20-08-2021 12 1   Download

Proximity ligation based techniques, like Hi-C, involve restriction digestion followed by ligation of formaldehyde cross-linked chromatin. Distinct chromatin states can impact the restriction digestion, and hence the visibility in the contact maps, of engaged loci.

15p vijeeni2711 24-07-2021 4 0   Download

In this paper, we propose an efficient, fast, powerful and stable improved hybrid algorithm in the Polar domain for eliminating the ring artifacts. This algorithm provides tight condensation of artifacts and hence preserves all the structure information in the image with reduced computational complexity. The results show that the superiority of new algorithm comparing with the existing techniques.

10p girlsseek 27-02-2019 25 0   Download

The Arabidopsis FAE1 b-ketoacyl-CoA synthase (FAE1 KCS) catalyzes the condensation of malonyl-CoA with longchain acyl-CoAs. Sequence analysis of FAE1 KCS predicted that this condensing enzyme is anchored to a membrane by two adjacent N-terminal membrane-spanning domains. In order to characterize the FAE1 KCS and analyze its mechanism, FAE1 KCS and its mutants were engineered with a His6-tag at their N-terminus, and expressed in Saccharomyces cerevisiae. The membrane-bound enzyme was then solubilized and purified to near homogeneity on a metal affinity column.

9p research12 01-06-2013 41 2   Download

The initial condensation event in the nonribosomal biosyn-thesisof thepeptideantibioticsgramicidinSandtyrocidineA takes place between a phenylalanine activating racemase GrsA/TycAand the ®rst proline-activatingmodule ofGrsB/ TycB. Recently we established a minimalin vitro model system for NRPS with recombinant His6-tagged GrsA (GrsA Phe-ATE; 127 kDa) and TycB1 (TycB1Pro-CAT; 120 kDa) and demonstrated the catalytic function of the C-domaininTycB1Pro-CAT to form a peptide bond between phenylalanine and proline during diketopiperazine formation (DKP). ...

10p research12 23-04-2013 31 1   Download

Condensin complexes are the key mediators of chromosome condensation. The MukB–MukE–MukF complex is a bacterial condensin, in which the MukB subunit forms a V-shaped dimeric structure with two ATPase head domains. MukE and MukF together form a tight complex, which binds to the MukB head via the C-terminal winged-helix domain (C-WHD) of MukF.

10p vinaphone15 25-02-2013 32 2   Download

Soft matter (or soft condensed matter) refers to a group of systems that includes polymers, colloids, amphiphiles, membranes, micelles, emulsions, dendrimers, liquid crystals, polyelectrolytes, and their mixtures. Soft matter systems usually have structural length scales in the region from a nanometer to several hundred nanometers and thus fall within the domain of “nanotechnology.” The soft matter length scales are often characterized by interactions that are of the order of thermal energies so that relatively small perturbations can cause dramatic structural changes in them.

0p thienbinh1311 13-12-2012 44 3   Download