Conformational epitope
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One of the major challenges in the field of vaccine design is identifying B-cell epitopes in continuously evolving viruses. Various tools have been developed to predict linear or conformational epitopes, each relying on different physicochemical properties and adopting distinct search strategies.
15p vikentucky2711 26-11-2020 16 1 Download
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The identification of immunogenic regions on the surface of antigens, which are able to be recognized by antibodies and to trigger an immune response, is a major challenge for the design of new and effective vaccines. The prediction of such regions through computational immunology techniques is a challenging goal, which will ultimately lead to a drastic limitation of the experimental tests required to validate their efficiency.
12p vioklahoma2711 19-11-2020 12 2 Download
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Anti-NMDA receptor encephalitis (ANRE) is a potentially lethal disease attributed to auto-antibodies against the N-methyl-D-aspartate receptor (NMDAR). Full recovery is possible if therapy is initiated early in the disease course. Detection of ANRE antibodies in the cerebrospinal fluid (CSF) is essential for diagnosis.
9p vihamax2711 21-04-2020 14 0 Download
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Cancer antigen CA125 represents an extracellular part of transmembrane mucin MUC16 and may be expressed in tissues of the male reproductive tract. It reaches human seminal plasma (hSP) after undergoing the main processes involved in protein speciation: Synthesis, posttranslational modifications, compartmentalization, and auto/proteolytic degradation.
9p nutifooddau 27-01-2019 15 1 Download
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The aim of the present study is to provide a strategy for predicting the production of antigenic regions of Clostridium novyi alpha toxin. The selection is based on B-cell epitopes and MHCII binding protein by immunoinformatics tools.
8p nutifooddau 27-01-2019 18 2 Download
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P-glycoprotein (Pgp), a membrane pump often responsible for the multidrug resistance of cancer cells, undergoes conformational changes in the presence of substrates/modulators, or upon ATP depletion, reflected by its enhanced reactivity with the UIC2 monoclonal antibody. When the UIC2-shift was elicited by certain modulators (e.g. cyclosporin A or vinblastine, but not with verapamil or Tween 80), the subsequent binding of other monoclonal anti-Pgp Ig sharing epitopes with UIC2 (e.g. MM12.10) was abolished [Nagy, H., Goda, K., Arceci, R., Cianfriglia, M., ´ Mechetner, E. & Szabo Jr, G.
6p system191 01-06-2013 59 5 Download
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Mucin glycoproteins on breast cancer cells carry shortened carbohydrate chains. These partially deglycosylatedmucin 1 (MUC-1) structures are recognized by the monoclonal antibody SM3, which is being tested for its diagnostic utility. We used NMR spectroscopy to analyze the binding mode and thebindingepitopeof peptideandglycopeptideantigens to the SM3 antibody. The pentapeptide PDTRP and the glycopentapeptide PDT(O-a-D-GalNAc)RP are known lig-ands of the monoclonal antibody.
12p research12 29-04-2013 38 2 Download
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Opacity-associated (Opa) proteins are outer membrane proteins which play a critical role in the adhesion of patho-genicNeisseriaspp. to epithelial and endothelial cells and polymorphonuclear neutrophils. The adherence is mainly mediated by the CD66-epitope-containing members of the carcinoembryonic-antigen family of human cell-adhesion molecules (CEACAM). For the analysis of the specific in-teractions of individual Opa proteins with their receptors, pure protein is needed in its native conformation. ...
9p tumor12 22-04-2013 30 1 Download
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Plasminogen activator inhibitor-1 (PAI-1) belongs to the serpin family of serine proteinase inhibitors. Serpins inhibit their target proteinases by an ester bond being formed between the active site serine of the proteinase and the P1 residue of the reactive centre loop (RCL) of the serpin, fol-lowed by insertion of the RCL intob-sheet A of the serpin. Concomitantly, there are conformational changes in the flexible joint region lateral tob-sheet A.
8p fptmusic 16-04-2013 27 1 Download
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Using NMR spectroscopy and simulated annealing calculations, we deter-mined the solution structure of the disulfide-linked cyclized decapeptide ACLPWSDGPC (SD), which is bound to an anti-(gibberellin A4) mAb 4-B8(8)⁄E9 and was found to be the first peptidyl mimotope for a hydro-phobic ligand. The resulting structure of the peptide showed ab-turn-like conformation in residues three to seven and the region converges well (average rmsd 0.54 A ˚ ).
11p fptmusic 12-04-2013 47 2 Download
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BacterialRNApolymerase (RNAP) is the central enzyme of gene expression that is responsible for the synthesis of all types of cellular RNAs. The process of transcription is accompanied by complex structural rearrangements of RNAP. Despite the recent progress in structural studies of RNAP, detailed mechanisms of conformational changes of RNAP that occur at different stages of transcription remain unknown.
11p awards 05-04-2013 39 1 Download
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Using solution NMR spectroscopy, three-dimensional structures have been obtained for an 18-residue synthetic polypeptide fragment of 18.5 kDa myelin basic protein (MBP, human residues Q81–T98) under three condi-tions emulating the protein’s natural environment in the myelin membrane to varying degrees: (a) an aqueous solution (100 mmKCl pH 6.5), (b) a mixture of trifluoroethanol (TFE-d2) and water (30 : 70% v⁄v), and (c) a dispersion of 100 mm dodecylphosphocholine (DPC-d38 , 1 : 100 pro-tein⁄lipid molar ratio) micelles....
14p dell39 27-03-2013 37 2 Download
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Conformational epitopes of myelin oligodendrocyte glycoprotein are targets of potentially pathogenic antibody responses in multiple sclerosis
9p panasonic09 12-01-2012 44 4 Download