Constituency to dependency

Microfibrils and elastin are major constituents of elastic fibers, the assembly of which is dictated by multimolecular interactions.Microfibril-associated glycoprotein-1 (MAGP-1) is a microfibrillar component that interacts with the sol-uble elastin precursor, tropoelastin. We describe here the adaptation of a solid-phase binding assay that defines the effect of divalent cations on the interactions between MAGP-1 and tropoelastin. Using this assay, a strong cal-cium-dependent interaction was demonstrated, with a dis-sociation constant of 2.8 ± 0.3 nM, which fits a single-site binding model.

0p awards 05-04-2013 42 1   Download