Construction of Plasmids

In this study, we evaluated the reliability of genomic DNAs and cloned recombinant plasmids as standard controls for absolute real-time PCR assay. The associated standard curves were constructed and used for the quantification of Lactobacillus acidophilus probiotics.

4p mudbound 10-12-2021 32 2   Download

An improved reverse genetics system for influenza A virus generation and its implications for vaccine production present an improved system for the generation of influenza viruses that allows the easy and repro ducible production of vaccine viruses in vero cells. Application of this system may be especially advantageous in situations of outbreaks of highly pathogenic avian influenza viruses.

5p thuyancn 01-06-2015 29 3   Download

A survey of plasmids for 51 prion protein constructs from bank vole, cat, cattle, chicken, dog, elk, ferret, frog, fugu, horse, human, pig, sheep, turtle, and wallaby, and for 113 mouse prion protein constructs and variants thereof, is presented. This includes information on the biochemistry of the recombinant proteins, in particular on successful and unsuccessful expres-sion attempts.

9p viettel02 22-02-2013 34 2   Download