Crystallization medium
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A new method for the synthesis of nanosized zeolites in organic solvents, such as formamide and toluene as crystallization medium instead of water, in the presence of organosilane has been developed. Organic solvents have a great impact on the synthesis of nanozeolites.
9p tuananhhumg 04-11-2013 54 3 Download
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The present study was conducted on human Jurkat T-cell lines in order to elucidate the role of phospholipase A2in capacitative calcium entry. We have employed thapsigar-gin (TG)that induces increases in [Ca 2+ ] i by emptying the calcium pool of endoplasmic reticulum, followed by capacitative calcium entry. We designed a Ca 2+ free/Ca 2+ reintroduction (CFCR)protocol for the experiments, conducted in Ca 2+ -free medium.
7p tumor12 22-04-2013 30 2 Download
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3-Hydroxyacyl-CoA dehydrogenase (HAD) functions in mitochondrial fatty acid b-oxidation by catalyzing the oxidation of straight chain 3-hyd-roxyacyl-CoAs. HAD has a preference for medium chain substrates, whereas short chain 3-hydroxyacyl-CoA dehydrogenase (SCHAD) acts on a wide spectrum of substrates, including steroids, cholic acids, and fatty acids, with a preference for short chain methyl-branched acyl-CoAs.
10p fptmusic 12-04-2013 34 3 Download
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Ouabain, a sodiumpump (Na + /K + -ATPase) inhibitor, has been shown to act as a hormone and is possibly involved in the pathogenesis of hypertension. The mechanismby which ouabain may act was investigated using primary cultures of human umbilical artery endothelial cells (HUAECs), which are known to express and release the vasoconstrictive hor-mone endothelin (ET-1). Fiveminutes after application, low concentrations of ouabain induced Ca 2+ oscillations and stimulated ET-1 release from endothelial cells into the medium.
9p dell39 03-04-2013 34 4 Download
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Acyl-CoA dehydrogenases constitute a family of flavopro-teins that catalyze the a,b-dehydrogenation of fatty acid acyl-CoA conjugates. While they differ widely in their spe-cificity, they share the same basic chemical mechanism ofa,b-dehydrogenation. Medium chain acyl-CoAdehydro-genase is probably the best-studiedmember of the class and serves as a model for the study of catalytic mechanisms.
15p dell39 03-04-2013 60 4 Download
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Structural analysis of protein tyrosine phosphatases (PTPs) has expanded considerably in the last several years, producing more than 200 structures in this class of enzymes (from 35 different proteins and their complexes with ligands). The small–medium size of the catalytic domain of280 resi-dues plus a very compact fold makes it amenable to cloning and over-expression in bacterial systems thus facilitating crystallographic analysis.
9p media19 05-03-2013 34 2 Download