Culture broth

Ophiocordyceps sinensis (O. sinensis) – commonly known as Dong Chong Xia Cao, is a medicinal fungus that contains valuable bioactive compounds such as cordycepin, polysaccharide…,in which, the exopolysaccharide (EPS) obtained from the cultured medium is a polysaccharide that has been convinced to have various bioactivities such as antioxidant, antibacterial, etc.

8p vipriyankagandhi 27-07-2022 4 2   Download

Cordyceps sp. CPA14V was isolated from insect-fungi sample that was collected from Copia - Son La Nature Reserve. The strain was able to biosynthesize cyclooligomer depsipeptides (CODs). This study aimed to identify Cordyceps sp. CPA14V to species using DNA sequence analysis and to estimate the effects of carbon, nitrogen, pH of culture broth on its growth and COD producing capacity.

7p vimelindagates 18-07-2022 10 2   Download

Seven compounds were isolated and characterized from the culture broth of the marine bacteria Nocardiopsis sp. (strain G057), which was isolated from sediment collecting at Cô Tô – Quảng Ninh. Their structures were determined by spectroscopic analysis including MS and 2D NMR, as well as by comparison with reported data in the literature. All compounds were evaluated for their antimicrobial activity against a panel of clinically significant microorganisms. Compounds 1, 2 and 7 selectively inhibited Escherichia coli with a MIC value of 32, 64, 8 μg/mL, respectively.

5p cothumenhmong11 10-05-2021 8 1   Download

Five secondary metabolites including three flavonoids, 6-lavandulyl-5,7,2′,4′-tetrahydroxyflavanone (1), 5′- lavandulyl-2′-methoxy-2,4,4′,6′-tetrahydroxychalcone (2), 6-lavandulyl-7-methoxy-5,2′,4′-trihydroxyflavanone (3), and two alkaloids, tryptoline (4), and norharman (5) were isolated from the culture broth of Streptomyces sp. C011. Their structures were determined by spectral data analysis, including MS, 2D NMR, and CD spectrum. All the separated compounds were evaluated for their antimicrobial activities. Compounds 1, 2, 3 and 5 exhibited antimicrobial activity...

5p cothumenhmong11 06-05-2021 13 2   Download

This study is aimed at developing microbiological culture media from sorghum grains in the cultivation of Staphylococcus aureus and Bacillus subtilis.

8p chauchaungayxua10 19-03-2021 11 2   Download

The present study was conducted to observe the effect of copper oxide nanoparticles in both solid and broth culture media against five fungal pathogens isolated from three crops growing in arid environments of Jodhpur.

9p chauchaungayxua11 23-03-2021 14 2   Download

In a recent study, we described two new lavandulylated flavonoids, along with eight known compounds from the culture broth of a Streptomyces sp. (strain G246), isolated from the sponge Halichondria panicea, collected in the sea of Son Tra peninsula (Da Nang). A comparison study was conducted to differentiate between solid and liquid fermentation technique for secondary metabolites production of strain G246. In this paper, we report the isolation and structural characterization of nine secondary metabolites (1-9) from strain G246 by solid state fermentation.

8p nguaconbaynhay10 19-02-2021 12 2   Download

The aim of this study was to evaluate the performance of an autotrophic denitrification during an anaerobic fermentation process usingH2/CO2 mixture gas (80:20, v/v) as sole energy source. Chemostat reactors were operated with anaerobically acclimated hydrogenotrophic methanogens culture broth.

5p cothumenhmong9 18-01-2021 10 2   Download

Alternaria helianthi the causal agent of sunflower leaf blight was isolated from a monoconidial culture derived from blight infected sunflower leaves of MSFH-8.Alternariahelianthi was first cultured on PDA and then transferred to Potato Dextrose broth. Toxin was purified from the cultural filtrate by treatment with organic solvents. The identity of the toxin was established by estimating carbohydrate and protein content. Based on the quantity of carbohydrate and protein the toxin was identified as glycoproteinaceous in nature.

5p gaocaolon9 22-12-2020 15 2   Download

This study was conducted to study the production and evaluation of potentially probiotic additives containing three live lactic bacteria culture broths, which could be used as a replacement for antibiotics in weaning and post-weaning piglets in Vietnam.

12p larachdumlanat127 20-12-2020 10 0   Download

In the present study, attempts for the isolation of Mycoplasma organisms were carried out in PPLO broth and PPLO agar medium. Out of 253 sheep and 112 goat nasal swab samples inoculated in PPLO broth, 27 sheep and 14 goat samples showed colour change in broth. Upon sub-culturing of 27 sheep and 14 goat broth nasal swab samples on PPLO agar, 17 sheep and 9 goat samples produced colonies.

7p trinhthamhodang1216 19-11-2020 15 2   Download

Bacterial canker of tomato caused by Clavibacter michiganensis subsp. michiganensis (Cmm) causes significant yield losses on tomatoes. It is newly observed bacterial disease in mid hills of Himachal Pradesh, India. Few or less work has, therefore, been done on this disease in the state. This study was conducted to find optimum growth conditions for the bacterium. Under present investigations, five isolates of Cmm were thus isolated and maintained in pure cultures.

7p angicungduoc8 07-11-2020 16 1   Download

In this study, we isolated and characterized an Enterococcus faecalis isolate from a diseased cultured Tilapia. Initial isolation of putative E. faecalis was carried out on streptococcus enrichment broth for 36 h. Characteristic, gram-positive, black color colony was selectively sub-cultured and subsequently identified by 16 rRNA sequencing analysis as Enterococcus faecalis (Genbank Acc no. KT877352).

11p trinhthamhodang1215 23-09-2020 12 1   Download

The study was conducted to describe the cultural characteristics such as colour and texture of the leaf blast pathogen Pyricularia oryzae (Cav.) on different solid media viz., potato dextrose agar, potato carrot agar, Kirchoff‟s, medium, Richard‟s medium, Sabourad‟s medium, Takahashii‟s medium, rice leaf extract agar and oat meal agar and liquid media viz., potato dextrose broth, potato carrot broth, Kirchoff‟s broth, Richard‟s broth, Sabourad‟s dextrose broth, Takahashii‟s broth and rice leaf extract broth.

7p trinhthamhodang1215 23-09-2020 7 1   Download

Maize Post flowering stalk rot causing caused by Fusarium verticillioides is major disease and causing yield loss up to 34 per cent. Different liquid media supporting the growth of the different isolates of fungus was observed. Among the eight-liquid media tested for the dry mycelial weight and sporulation of six isolates of Fusarium stalk rot pathogen (F. verticillioides), Isolate FV-3 yielded highest dry mycelial weight of 787.66mg, followed by FV-2 (647.66mg), FV1(581.00mg), FV-5 (451.00mg), FV-6(421.00mg) and FV7(277.66mg) in Takahashii’s broth.

6p caygaocaolon7 18-09-2020 16 0   Download

The objective of this study is to isolate and screen fungal strains with antibacterial activity from the marine environment. Twenty five strains of fungi were isolated from marine sediments of Thanh Lan, Co To island and assessed on antibiotic activity against 7 tested microbial strains, including three Gram-negative bacteria (Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853, Salmonella enterica ATCC13076), three Gram-positive bacteria (Enterococcus faecalis ATCC29212, Stapphylococus aureus ATCC25923, Bacillus cereus ATCC 13245), and the yeast Candida albicans ATCC10231.

8p nguyenxuankha_bevandan 14-08-2020 38 4   Download

Black banded disease of mango, caused by Peziotrichum corticolum (Massee) Subramanian, is becoming severe for the last three to four years in all the mango growing regions. Morphological and cultural characteristics of the pathogen were studied. The identity of the fungus was confirmed. The fungus showed maximum radial growth on host extract dextrose agar on 12th day after incubation at 27±1oC. Culture of P. corticolum exhibited diversity with respect to cultural characters like type of the growth, mycelial colour, pigmentation and colony margin.

11p trinhthamhodang6 09-07-2020 11 1   Download

Morphological and cultural variability of S. rolfsii infecting tomato were studied on different solid and liquid media based on radial growth, topography, sclerotial number, diameter of sclerotia, test weight of sclerotial bodies and dry mycelial weight. Significant variability with reference to mycelial and sclerotial characters was observed on different media. This investigation revealed that the maximum mycelial growth was observed in Potato Dextrose Agar (9 cm) and more test weight (262 mg) of sclerotial bodies was recorded in Sabouraud’s dextrose agar.

8p chauchaungayxua6 26-06-2020 7 2   Download

Effect of different temperature and culture media was observed on all ten Trichoderma viride isolates. The excellent growth of Trichoderma was found at temperature range of 25–30˚C. All the 10 Trichoderma isolates were evaluated on four different liquid media for assessing growth and sporulation. The maximum mycelial weight was found in potato dextrose broth (276.00 mg) and the lowest Czapek Dox broth (96.00 mg).

4p nguaconbaynhay6 24-06-2020 10 1   Download

This paper presents an in vitro experiment conducted to evaluate the optimum temperature and pH for growth of Phaeoacremonium parasiticum, responsible for oleoresin deposition in agarwood trees. In the present study, P. parasiticum was grown at four different levels of temperature viz., 25 ± 1°C, 27 1°C, 30 ± 1°C and 32 ± 1°C, and it was found to attain the highest radial growth at 30 ± 1°C (5.3 cm) followed by that attained at 32 ± 1°C (0.0452 m), 27 ± 1°C (0.0450 m) and 25 ± 1°C (0.0240 m). P. parasiticum was also grown at five different levels of pH viz., 5.0, 5.5, 6.0, 6.5 and 7.

8p nguaconbaynhay6 24-06-2020 12 0   Download