Curve resolution
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Bangladesh lies in the global thalassemia belt, which has a defined mutational hot-spot in the betaglobin gene. The high carrier frequencies of beta-thalassemia trait and hemoglobin E-trait in Bangladesh necessitate a reliable DNA-based carrier screening approach that could supplement the use of hematological and electrophoretic indices to overcome the barriers of carrier screening.
12p vinarcissa 21-03-2023 2 1 Download
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Like glucose-6-phosphate dehydrogenase (G6PD) deficient hemizygous males and homozygous females, heterozygous females could also manifest hemolytic crisis, neonatal hyperbilirubinemia or kernicterus upon exposure to oxidative stress induced by certain foods such as fava beans, drugs or infections.
9p vihagrid 30-01-2023 7 3 Download
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Current biopharmaceutical production heavily relies on chromatography for protein purification. Recently, research has intensified towards finding suitable solutions to monitoring the chromatographic steps by multivariate spectroscopic sensors.
9p viginny 23-12-2022 6 2 Download
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In this study, non-targeted gas chromatography-Orbitrap-mass spectrometry (GC-Orbitrap-MS) analysis of semi-volatile organic compounds (SVOCs) in indoor environmental dust samples is proposed. Highresolution mass spectrometry (HRMS) provides massive amounts of information-rich mass data which presents storage and processing challenges.
23p viginny 23-12-2022 9 3 Download
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This study aimed to propose a mathematical method to investigate and optimize the simultaneous elimination process of multiple organic pollutants using the Fenton process. Hence, the treatment of rhodamine B (RB) and acid red 14 (AR14) dyes in their binary solution was studied. Multivariate curve resolution alternating least square (MCR-ALS), a novel chemometric method, was applied along with correlation constraints to resolute the UV-Vis spectrophotometric data, enabling quantification of investigated dyes despite a high spectral overlapping.
15p tudichquannguyet 29-11-2021 10 1 Download
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The PCR technique and its variations have been increasingly used in the clinical laboratory and recent advances in this field generated new higher resolution techniques based on nucleic acid denaturation dynamics.
14p vikentucky2711 26-11-2020 3 1 Download
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The collection and analysis of Atomic Force Microscopy force curves is a well-established procedure to obtain high-resolution information of non-topographic data from any kind of sample, including biological specimens.
12p viconnecticut2711 28-10-2020 11 2 Download
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Primary small cell carcinoma of the esophagus (PSCCE) is a rare and aggressive tumor with poor prognosis. The aim of this study was to investigate the existence of EGFR, KRAS, PIK3CA and PTEN mutations in PSCCE. Methods: Clinical–pathological data and paraffin-embedded specimens were collected from 38 patients. Exons 18 to 21 of EGFR, KRAS and PIK3CA status were analyzed by real-time PCR based on ARMS and Scorpion technology in all patients, and the PTEN gene was also screened using real-time PCR and high-resolution melting curve analysis (HRMA).
8p vijennie2711 25-09-2020 18 2 Download
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High resolution melting curve analysis is a cost-effective rapid screening method for detection of somatic gene mutation. The performance characteristics of this technique has been explored previously, however, analytical parameters such as limit of detection of mutant allele fraction and total concentration of DNA, have not been addressed.
12p virose2711 25-09-2020 31 0 Download
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A novel simple, direct and selective gas chromatography–mass spectrometry (GC/MS) procedure was developed for the determination of the antihistamine drug dimenhydrinate (DMH) in presence of six of its related substances and potential impurities, namely, diphenylmethane, diphenylmethanol, benzophenone, orphenadrine, caffeine and 8-chlorocaffeine. The method involved resolution of the underivatized compounds using a trifluoropropylmethyl polysiloxane (Rtx-200) capillary column and the mass spectrometric detection was carried out in the electronimpact (EI) mode.
6p kequaidan1 16-11-2019 16 0 Download
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Multivariate Curve Resolution with Alternating Least Squares (MCR-ALS) is a curve resolution method based on a bilinear model which assumes that the observed spectra are a linear combination of the spectra of the pure components in the system. The algorithm steps include the determination of the number of components by rank analysis methods, initial estimates for the concentrations and/or spectra and an iterative optimization. Sometimes, suitable results may not be achieved when MCR-ALS is applied. One reason for this is the importance of the initial estimates of the spectral profiles.
8p kequaidan1 16-11-2019 16 0 Download
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This paper presents a study of the properties of sounding curves equivalent to the different electrode arrays by means of simulations for numerical modeling. Following this, the authors proposed a calculation for the synthetical apparent resistivity values in effort to gain higher-resolution sounding curves in electric sounding measurement.
5p cathydoll4 21-02-2019 25 0 Download
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The present paper is devoted to examine the interaction between parametric and forced oscillations in fundamental resonances. Some remark about the resolution of the equations determining the stationary oscillations will begiven, some particularities of the resonance curve will be described.
8p bautroibinhyen211 28-10-2018 28 1 Download
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Eukaryotic genomes are composed of isochores, i.e. long sequences relat-ively homogeneous in GC content. In this paper, the isochore structure of Arabidopsis thalianagenome has been studied using a windowless technique based on theZcurve method and intuitive curves are drawn for all the five chromosomes. Using these curves, we can calculate the GC content at any resolution, even at the base level.
9p fptmusic 11-04-2013 39 2 Download
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Radioactive sources which used to study were the standardized radioactive of common monoenergetic radionuclide Cs137 and standardized multigamma Co60, Ba133, Eu152. Determination of the absolute efficiency curve and energy resolution as a function of energy of GEM40P4 HPGe spectrometer are presented in this paper.
6p tuanlocmuido 19-12-2012 41 3 Download
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Velocity encoded phase-contrast magnetic resonance imaging (VE-PC MRI) is another imaging technique used to noninvasively measure Achilles tendon strain and changes in its force-displacement relationship concomitant with chronic unloading and subsequent recuperation. This technique will be reviewed in terms of its ability to quantify the Achilles tendon Young’s modulus (MPa) from a stress-strain curve.
206p wqwqwqwqwq 21-07-2012 62 5 Download