Determination of protease

Alpha-1 antitrypsin (A1AT) is one of the acute phase proteins which are synthesized in the liver. A1AT inhibits the activity of many proteases, but its main task is to protect the lungs from the attack of neutrophil elastase. In an autosomal hereditary disease known as alpha-1 antitrypsin deficiency, the A1AT level in blood serum decreases, increasing the risk of developing emphysema, liver apoptosis, and liver cancer. Thus, the detection of A1AT concentration in blood serum is very important.

15p tudichquannguyet 29-11-2021 6 1   Download

The stabilization and three-dimensional modeling of mutant proteins were also determined by using the I-Mutant 2.0 and Project HOPE webservers, respectively. In total, 772 missense SNPs were found in the APAF1 gene from the NCBI dbSNP database, 18 SNPs of which were demonstrated to be deleterious or damaging. Of those, 13 SNPs had a decreasing effect on protein stability, while the other 5 SNPs had an increasing effect. Based on the modeling results, some dissimilarities of mutant type amino acids from wild-type amino acids such as size, charge, and hydrophobicity were revealed.

11p thiencuuchu 27-11-2021 12 3   Download

Papain-like cysteine proteases (PLCPs), a large group of cysteine proteases structurally related to papain, play important roles in plant development, senescence, and defense responses. Papain, the first cysteine protease whose structure was determined by X-ray crystallography, plays a crucial role in protecting papaya from herbivorous insects. Except the four major PLCPs purified and characterized in papaya latex, the rest of the PLCPs in papaya genome are largely unknown.

12p vilarryellison 29-10-2021 6 0   Download

This study was proposed to determine the recovery rate of Aspergillus species in Ankara, Turkey, from soil and their identification as well as to evaluate the proteolytic activity of the isolated strains. 20 different soil samples were collected and searched for the presence of the fungal species after selective plating and internal transcribed spacer (ITS) gene region sequencing of cPCR fragments.

9p cothumenhmong11 11-05-2021 13 1   Download

Napsin A, an aspartic protease, is mainly expressed in alveolar type-II cells and renal proximal tubules and is a putative immunohistochemical marker for pulmonary adenocarcinomas. This study sought to determine whether napsin A could be measured in the serum to evaluate its relationship to idiopathic pulmonary fibrosis (IPF) and determine whether renal dysfunction might affect serum napsin A levels.

7p vianthony2711 16-04-2021 9 2   Download

The aim of the present investigation was to study the effect of soybean leaf protein and protease inhibitor content on the mean leaf area consumed by Spodoptera litura and Spilosoma obliqua larva. The activity of trypsin inhibitor was assayed by determining the residual trypsin activity by using BapNA as the substrate and bovine trypsin as the standard enzyme.

10p cothumenhmong9 18-01-2021 9 2   Download

Detection and screening of genes related to biosynthesis of substances with protease inhibitory activity from microorganisms associated with sponge collected from the Mien trung sea of Vietnam by metagenomics method. Then, selective gene, expression of in vitro and determination of activity of the expressed protein.

27p capheviahe26 25-01-2021 13 3   Download

The aim of this study was to examine the effect of UV irradiation on alkaline protease gene expression and induction of overproducing strains. We aimed also to determine the genetic variation among high protease productive mutants using RAPD-PCR. The promising strains in this study may be used in various economic industrial applications and for successive genetic improvement as well.

12p gaocaolon9 22-12-2020 12 2   Download

High performance liquid chromatography was applied for the determination of tryptophan in food. The sample was hydrolyzed in a duration from 16 to 24 hour by protease enzyme at 50o C. The extract was separated on the C8 reversed phase column with mobile phase of NaH2PO4 50mM pH 2.3: Methanol (82:18; v/v). The linearity of the method was kept in the range of 0.5 - 50 mg/L. Limit of detection was found to be 4.6 mg/100g.

7p kethamoi9 01-12-2020 19 1   Download

In this paper, we report potential application of bromelain isolated from pineapple shoots to design an experiment for teaching Practical Biochemistry. Bromelain from ethanolic fraction of pineapple shoots was used to design experiments related to enzyme and protein such as determining the isoelectric point of the protein, determining the activity of protease, electrophoresis, and enzyme kinetics.

7p tamynhan9 02-12-2020 20 1   Download

Alkaline keratinolytic protease producing Bacillus species was screened out from soil sample and characterized by studying morphological, cultural and biochemical properties. It was identified by using Burgey‟s manual of determinative bacteriology, identification flow charts as Bacillus alcalophilus. The isolate was employed for the production of alkaline protease by using production medium consisting of glucose and casein as carbon and nitrogen sources at pH 8.5 (Rao and Narasu, 2007).

15p dayhoctainha 10-09-2020 20 1   Download

Microbial enzymes have significant biotechnological application in industries. The purpose of this study was to isolate and identify microorganisms associated with ripe plantain fruits, determine the enzyme activity (lipase, protease, pectinase, cellulase and amylase) of the ripe plantain fruits samples and screen the isolated microorganisms for enzyme production.

16p nguaconbaynhay7 15-08-2020 14 2   Download

The filamentous fungus Paecilomyces is currently being developed as a biocontrol agent against plant parasitic nematodes, which often cause black leaf disease in pepper and coffee trees. Nematode eggs and cuticles are the infection sites for biocontrol agents that penetrate those areas by the production of lytic enzymes. Paecilomyces variotii NV01 was isolated from pepper soil from the Dak Lak province. The purpose of this work is to study the activity of protease and chitinase in the strain Paecilomyces variotii NV01. The protease activity was determined to be 82.3 U/ml in a 0.

6p chauchaungayxua2 07-01-2020 30 0   Download

Midgut hydrolytic enzymes of Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) were identified and partially characterized. Km, Vmax, optimum pH, and specific activity were determined for proteolytic enzymes and αamylases.

10p masterbarista 15-01-2019 17 1   Download

Response surface methodology (RSM) was used to determine optimal condition for continuous overflow proteolytic hydrolysis of spent brewer’s yeast. The optimal conditions for obtaining high degree of hydrolysis and low bitterness are determined as followings: ratio of enzyme mixture (alcalase 7.5 U/g and flavourzyme 10 U/g), pH at 7.5, hydrolysis temperature at 51o C and hydrolysis time of 9 hours. Under the optimal conditions, the yield of hydrolysis was 59.62% ± 0.027 and the bitterness equivalently with concentration of quinine was 7.86 ± 0.033 µmol /ml.

8p nguyenhong1235 28-11-2018 53 0   Download

Human glandular kallikrein 2 (hK2) is a trypsin-like serine protease expressed predominantly in the prostate epithelium. Recently, hK2 has proven to be a useful marker that can be used in combination with prostate specific antigen for screening and diagnosis of prostate cancer. The cleavage by hK2 of certain substrates in the proteolytic cascade suggest that the kallikrein may be involved in prostate cancer development; however, there has been very little other progress toward its biochemical characterization or elucidation of its true physiological role.

8p system191 01-06-2013 41 1   Download

Even though more than 200 three-dimensional structures of HIV-1 protease complexed to a variety of inhibitors are available in the Protein Data Bank; very few struc-tures of unliganded protein have been determined. We have recently solved structures of unliganded HIV-1 protease tethered dimer mutants to resolutions of 1.9 A˚ and 2.1 A ˚ , and have found that the flaps assume closed-flap conformation even in the absence of any bound lig-and.

9p tumor12 20-04-2013 20 1   Download

The substrate specificity of the nuclear inclusion protein a (NIa) proteolytic enzymes from two potyviruses, the tobacco etch virus (TEV) and tobacco vein mottling virus (TVMV), was compared using oligopeptide substrates. Mutations were introduced into TEV protease in an effort to identify key determinants of substrate specificity. The specificity of the mutant enzymes was assessed by using peptides with complementary substitutions. The crys-tal structure of TEV protease and a homology model of TVMV protease were used to interpret the kinetic data....

0p awards 05-04-2013 32 1   Download

ATP-dependent Lon proteases belong to the superfamily of AAA + proteins. Until recently, the identity of the residues involved in their proteolytic active sites was not elucidated. However, the putative catalytic Ser–Lys dyad was recently suggested through sequence comparison of more than 100 Lon proteases from various sources. The presence of the catalytic dyadwas experimentally confirmedby site-directed mutagenesis of theEscherichia coliLon protease and by determination of the crystal structure of its proteolytic domain.

0p awards 05-04-2013 34 1   Download

TheCPBgenes of the protozoan parasiteLeishmania mex-icana encode stage-regulated cathepsin L-like cysteine pro-teases that are important virulence factors and are in a tandem array of 19 genes. In this study, we have compared the substrate preferences of two CPB isoforms, CPB2.8 and CPB3, and a H84Y mutant of the latter enzyme, to analyse the roles played by the few amino acid differences between the isoenzymes in determining substrate specificity. CPB3 differs fromCPB2.8 at just three residues (N60D,D61Nand D64S) in the mature domain. ...

0p awards 05-04-2013 35 1   Download