DNA fragmentation
-
Many genetic/genomic disorders are caused by genomic rearrangements. Standard methods can often characterize these variations only partly, e.g., copy number changes or breakpoints. It is important to fully understand the order and orientation of rearranged fragments, with precise breakpoints, to know the pathogenicity of the rearrangements.
17p vibransone 28-03-2024 3 2 Download
-
Despite a myriad of attempts in the last three decades to diagnose ovarian cancer (OC) earlier, this clinical aim still remains a significant challenge. Aberrant methylation patterns of linked CpGs analyzed in DNA fragments shed by cancers into the bloodstream (i.e. cell-free DNA) can provide highly specific signals indicating cancer presence.
14p vioraclene 31-03-2024 3 1 Download
-
The fine-scale cell-free DNA fragmentation patterns in early-stage cancers are poorly understood. We developed a de novo approach to characterize the cell-free DNA fragmentation hotspots from plasma whole-genome sequencing.
15p vicwell 29-02-2024 2 1 Download
-
This study aimed to develop a method using DNA barcode fragments to identify the hybrid Eucalyptus UP99 and UP95. The total genomic DNA was extracted from leaf samples of UP99 and UP95 and was used to amplify the DNA barcodes (matK, rbcL, trnH-psbA, ITS and ITS2) by PCR.
11p vikissinger 21-12-2023 6 1 Download
-
Part 1 book "Environmental microbiology - Methods and protocols" includes content: Methods for isolation and cultivation of filamentous fungi, rapid extraction of PCR-competent DNA from recalcitrant environmental samples, quantitative PCR for detection of mRNA and gDNA in environmental isolates, analysis of community dynamics in environmental samples using denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphism (T-RFLP) profiling of bacterial 16S rRNA genes,... and other contents.
134p oursky10 04-12-2023 4 2 Download
-
Late detection of hepatocellular carcinoma (HCC) results in an overall 5-year survival rate of less than 16%. Liquid biopsy (LB) assays based on detecting circulating tumor DNA (ctDNA) might provide an opportunity to detect HCC early noninvasively.
17p vischultz 20-10-2023 3 1 Download
-
To unravel how the integrity of nuclear and mitochondrial circulating cell-free DNA (cfDNA) contributes to its plasma quantity in colorectal cancer (CRC) patients. Methods CfDNA from plasma samples of 80 CRC patients stratified by tumour stage and 50 healthy individuals were extracted. Total cfDNA concentration was determined and equal template concentrations (ETC) were analyzed by quantitative real-time PCR (qPCR) resulting in small and long fragments of KRAS, Alu and MTCO3.
14p vischultz 20-10-2023 4 1 Download
-
We present a case of sporotrichosis diagnosed by molecular biology combined with traditional methods. Fungal DNA was extracted from skin tissues of the patient by fungal DNA extraction kit. The PCR amplified fragments were compared on GenBank website, and the pathogenic fungi were identified.
4p viintuit 26-09-2023 1 0 Download
-
The new species is clearly distinguished from its congener Weygoldtia davidovi (Fage, 1946) by having more articles in the tibia and tarsus of leg I. A fragment of the cytochrome c oxidase subunit I (COI) gene is also provided for the new species. The K2P genetic distance of the COI between the new species and W. davidovi is 20%, and phylogenetic analysis also supported the new species.
10p vicaptainmarvel 21-04-2023 4 2 Download
-
Bài viết Ảnh hưởng của đứt gãy DNA tinh trùng đến kết quả IVF/ICSI đánh giá mối liên quan giữa mức độ đứt gãy DNA (DFI- DNA fragmention index) của tinh trùng với kết quả của IVF/ICSI của 82 cặp vợ chồng vô sinh.
5p viantman 05-04-2023 6 4 Download
-
Determination of microsatellite lengths or other DNA fragment types is an important initial component of many genetic studies such as mutation detection, linkage and quantitative trait loci (QTL) mapping, genetic diversity, pedigree analysis, and detection of heterozygosity.
8p vinarcissa 21-03-2023 4 1 Download
-
Canine and wolf mitochondrial DNA haplotypes, which can be used for forensic or phylogenetic analyses, have been defined in various schemes depending on the region analyzed. In recent studies, the 582 bp fragment of the HV1 region is most commonly used. 317 different canine HV1 haplotypes have been reported in the rapidly growing public database GenBank.
7p vinarcissa 21-03-2023 3 1 Download
-
Double minute chromosomes are circular fragments of DNA whose presence is associated with the onset of certain cancers. Double minutes are lethal, as they are highly amplified and typically contain oncogenes. Locating double minutes can supplement the process of cancer diagnosis, and it can help to identify therapeutic targets.
7p vinarcissa 21-03-2023 3 1 Download
-
In a deep sequencing analysis of small RNAs prepared from a living fossil, the tadpole shrimp Triops cancriformis, a 32-nt small RNA was specifically detected in the adult stage. A nucleotide sequence comparison between the 32-nt small RNA and predicted tRNA sequences in the draft nuclear genomic DNA showed that the small RNA was derived from tRNAGly(GCC).
12p vinarcissa 21-03-2023 3 1 Download
-
Grass carp (Ctenopharyngodon idellus, GC), as the highest-output fish in China, is economically important. The production of gynogenetic grass carp (GGC) will provide important germplasm resource for producing improved GC. At present, knowledge regarding the heterologous sperm DNA in gynogenetic offspring is little.
7p vihagrid 30-01-2023 4 3 Download
-
Two individuals with a first-degree relationship share about 50 percent of their alleles. Parent–offspring relationships cannot be homozygous for alternative alleles (genetic exclusion). Methods: Applying the concept of genetic exclusion to HD arrays typed in animals for experimental purposes or genomic selection allows estimation of the rate of rejection of first-degree relationships as the rate at which two individuals typed for a large number of Single Nucleotide Polymorphisms (SNPs) do not share at least one allele.
12p vihagrid 30-01-2023 8 3 Download
-
Protein purification by affinity chromatography relies primarily on the interaction of a fused-tag to the protein of interest. Here, we describe a tag-free affinity method that employs functional selection interactions to a broad range of proteins.
9p viginny 23-12-2022 12 3 Download
-
DNA barcoding is a short DNA fragment, is used to distinguish between species, especially morphologically similar species and processed specimens that are difficult to distinguish species. For plants, the regions of DNA used as barcodes in identification or taxonomy are usually sequences of the chloroplast genome and the nuclear genome, including the coding regions and the non-coding regions.
9p vilamborghini 12-10-2022 8 4 Download
-
Kurniwan et al. (2010) specified the distribution of F. cancrivora to the south of the Isthmus of Kra and a large area in the North of the Isthmus of Kra, including Vietnam, is the distribution range of F. moodiei, but the samples analyzed in that study did not include samples collected from Vietnam. Based on the results of morphological and genetic analyses on two mitochondrial gene fragments (16S and Cytb) of populations of Fejervarya moodiei and F. cacrivora, this study confirmed that the Northern Crab-eating Frog F. moodiei distributed in Vietnam.
11p vikoenigsegg 26-09-2022 21 3 Download
-
Nucleosomes consist of DNA wrapped around a histone octamer core like thread on a spool to condense DNA as chromatin into chromosomes. Diseases such as cancer or inflammation lead to cell death, chromatin fragmentation and release of nucleosomes into the blood.
11p vidarwin 23-02-2022 13 1 Download